Primer trabajo a nivel mundial sobre la diferenciación de especies de Pinus con clave dicotómica a través de la estructura cuticula

Garcia Alvarez, Salvia

Garcia Amorena, Ignacio

Morla Juaristi, Carlos

Paull, Rosemary

Botanical Journal of the Linnean Society

Servicio de Coordinación de Bibliotecas de la Universidad Politécnica de Madrid

A taxonomic tool for identifying needle remains of south-western European Pinus species of the Late Quaternary

International audienceThis work provides a tool whereby the needle remains of native, southwestern European Pinus spp. can be easily identified from species-specific epidermal features. To construct this tool, the needles of P. uncinata, P. sylvestris, P. nigra, P. pinaster, P. pinea and P. halepensis were gathered across the Northern Hemisphere range of each taxon and compared with non-indigenous trees growing in two South Australian Botanic Gardens. Three needles from each of these species were taken from three adult trees growing at three different localities. Light microscopy was used to observe the key epidermal and stomatal features of the needles. To improve interpretation, additional scanning electron microscopy samples were prepared. Epidermal features, including variation in the diameter of the epistomatal chamber aperture (pore), are described. A taxonomic key based on the size, shape and arrangement of the subsidiary cells of the stomatal complexes was constructed. This key enables the identification of pine needle fragments at the species level (except those belonging to the group P. gr. nigra-uncinata). Despite their overlapping range, pore size was helpful in distinguishing between P. nigra and P. uncinata and between three groups of species. Isolated stomata were also observed. Cluster and discriminant analyses of stomatal variables described in earlier studies were performed. Overlap in guard cell variables hampers species-level identification of isolated stomata. Species discrimination is improved if groups of ecological affinity are considered

García Álvarez, Salvia

García-Amorena, Ignacio

Archive Ouverte en Sciences de l'Information et de la Communication

Archivo Digital UPM

This work provides a tool whereby the needle remains of native, south-western European Pinus spp. can be easily identified from species-specific epidermal features. To construct this tool, the needles of P. uncinata, P. sylvestris, P. nigra, P. pinaster, P. pinea and P. halepensis were gathered across the Northern Hemisphere range of each taxon and compared with non-indigenous trees growing in two South Australian Botanic Gardens. Three needles from each of these species were taken from three adult trees growing at three different localities. Light microscopy was used to observe the key epidermal and stomatal features of the needles. To improve interpretation, additional scanning electron microscopy samples were prepared. Epidermal features, including variation in the diameter of the epistomatal chamber aperture (pore), are described. A taxonomic key based on the size, shape and arrangement of the subsidiary cells of the stomatal complexes was constructed. This key enables the identification of pine needle fragments at the species level (except those belonging to the group P. gr. nigra-uncinata). Despite their overlapping range, pore size was helpful in distinguishing between P. nigra and P. uncinata and between three groups of species. Isolated stomata were also observed. Cluster and discriminant analyses of stomatal variables described in earlier studies were performed. Overlap in guard cell variables hampers species-level identification of isolated stomata. Species discrimination is improved if groups of ecological affinity are considered

This work provides a tool whereby the needle remains of native, south-western European Pinus spp. can be easily

identified from species-specific epidermal features. To construct this tool, the needles of P. uncinata, P. sylvestris,

P. nigra, P. pinaster, P. pinea and P. halepensis were gathered across the Northern Hemisphere range of each

taxon and compared with non-indigenous trees growing in two South Australian Botanic Gardens. Three needles

from each of these species were taken from three adult trees growing at three different localities. Light microscopy

was used to observe the key epidermal and stomatal features of the needles. To improve interpretation, additional

scanning electron microscopy samples were prepared. Epidermal features, including variation in the diameter of

the epistomatal chamber aperture (pore), are described. A taxonomic key based on the size, shape and arrangement

of the subsidiary cells of the stomatal complexes was constructed. This key enables the identification of pine needle

fragments at the species level (except those belonging to the group P. gr. nigra-uncinata). Despite their overlapping

range, pore size was helpful in distinguishing between P. nigra and P. uncinata and between three groups of

species. Isolated stomata were also observed. Cluster and discriminant analyses of stomatal variables described in

earlier studies were performed. Overlap in guard cell variables hampers species-level identification of isolated

stomata. Species discrimination is improved if groups of ecological affinity are considered

HAL Clermont Université

Abstract not availableSalvia García Álvarez, Carlos Morla Juaristi, Rosemary Paull and Ignacio García-Amoren

A taxonomic tool for identifying needle remains of south-western European Pinus species of the Late Quaternary

Abstract

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Full text

Available Versions

Servicio de Coordinación de Bibliotecas de la Universidad Politécnica de Madrid

Archive Ouverte en Sciences de l'Information et de la Communication

Archivo Digital UPM

Archivo Digital UPM

Servicio de Coordinación de Bibliotecas de la Universidad Politécnica de Madrid

HAL Clermont Université

Adelaide Research & Scholarship

HAL-UNILIM

A taxonomic tool for identifying needle remains of south-western European Pinus species of the Late Quaternary

Abstract

Similar works

Full text

Available Versions

Servicio de Coordinación de Bibliotecas de la Universidad Politécnica de Madrid

Archive Ouverte en Sciences de l'Information et de la Communication

Archivo Digital UPM

Archivo Digital UPM

Servicio de Coordinación de Bibliotecas de la Universidad Politécnica de Madrid

HAL Clermont Université

Adelaide Research &amp; Scholarship

HAL-UNILIM

Adelaide Research & Scholarship