Bartaloni, J.

Bigi, A.

Brandi, M.L.

Carossino, A.M.

Facchini, A.

Galli, G.

Gozzini, A.

Mavilia, C.

Recenti, R.

Tanini, A.

Tognarini, I.

Zonefrati, R.

Scientific Open-access Literature Archive and Repository

DIFFERENTIATION TOWARD OSTEOGENESIS OF STEM CELLS FROM ADIPOSE TISSUE AND BONE MARROW: INTERACTIONS WITH NANOSTRUCTURED BIOMATERIALSI. Tognarini1, J. Bartaloni1, G. Galli1, R. Zonefrati1, R. Recenti1, A.M. Carossino1, C. Mavilia1, A. Gozzini1, A. Tanini1, A. Bigi2, A. Facchini3, M.L. Brandi11 Department of Internal Medicine, University of Florence, Florence, Italy2 Department of Chemistry, University of Bologna, Bologna, Italy3 LIMA-LTO S.p.A.Cell therapy realized by tissue engineering and expansion “ex vivo” of various cell types, has had applica-tions in immunotherapy, organ transplant and renewal of tissues. To regenerate bone tissue are usedtechniques that provide the cell growth on biosynthetic materials suitable for stimulate and induct the os-teogenesis. These materials are xenobiotic products, as alloys of Ti/Al/V or Ti/Nb/Zr, natural products asthe hydroxyapatite (HA). In our study we evaluated the differentiation toward osteogenesis of the adiposetissue and bone marrow derived stem cell cultured on nanostructured biomaterials. Our research are focused about the differentiation toward osteogenesis of adipose tissue and bone mar-row derived stem cells cultured on four biomaterials: alloy of Ti/Al/V, nanostructured alloy of Ti/Nb/Zr andthe same two alloys coated by hydroxyapatite. Therefore, we carried out, using the above mentionedstem cells, the immunoenzymatic alkaline phosphatase determination and the quantitative analysis (RT-PCR) of the genetic expression of the most significant osteoblastic markers during all the differentiationperiod. The cellular growth of the adipose tissue and bone marrow derived stem cell is better on two naked alloysthan that on the same alloys coated by hydroxyapatite, however, are never revealed toxic phenomena.On the four biomaterials are maintained during all the differentiation period the osteoblastic phenotypewith comparable results between primary coltures from adipose tissue and bone marrow. Therefore,these biomaterials seem to be effectiveness to the differentiation toward osteoblastic cell type of the adi-pose tissue and bone marrow derived stem cell.Clinical Cases in Mineral and Bone Metabolism 2005; 2(3): 185-252 247BSTR CT (V Congresso CCMBM)  18/11/2005  15.59  Pagina 247FOR REVIEW ONLY© CIC EDIZIONI INTERNAZIONALI

Differentation toward osteogenesis of stem cells from adipose tissue and bone marrow: interactions with nanostructured biomaterials

http://eprints.bice.rm.cnr.it/9153/1/article.pdf

Differentation toward osteogenesis of stem cells from adipose tissue and bone marrow: interactions with nanostructured biomaterials

Abstract

Similar works

Full text

Available Versions

Scientific Open-access Literature Archive and Repository