25 p.This study was carried out to determine the occurrence and characteristics of enteropathogenic
18 Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) strains in cow’s milk, cheese
19 and dairy cattle farm environments, and to estimate distribution of antimicrobial resistance. A
20 collection of 18 atypical EPEC -aEPEC, 14 STEC, and one E. albertii was obtained and
21 characterized from 502 samples. Occurrence of aEPEC in cow’s milk was high (>6%) whereas non-
22 O157 STEC was isolated in ca. 2% of milk samples. Detection of these diarrheagenic E. coli was absent in more than 100 cheese samples obtained from raw milk. This is the first 23 report identifying
24 E. albertii (O69:HNM) in a dairy cattle farm. Nearly one-third of aEPEC strains showed
25 antimicrobial resistance, mostly presenting a multidrug resistance pattern. One clonal complex
26 (ST20 Cplx) containing aEPEC strains from milk and faecal samples was determined. Two STEC
27 strains belonged to serotypes with importance in human disease (O91:H21 and O55:H8) and were
28 isolated from air samples which suggests a high dissemination potential. Spanish bulk tank cow’s
29 milk can constitute an important source of aEPEC strains besides STEC, bearing multiple
30 antimicrobial resistance and with high diversity of both serotypes and genetic features linked to
31 potential human infectionS
