This study evaluated the enzymatic conversion of alkaline peroxide oxidative pretreatment of an invasive lignocellulosic biomass (siam weed) to reducing sugar, amenable to further microbial effects at the downstream processing. Using a statistical design of experiments approach (response surface methodology), optimum pretreatment conditions of 43.7 oC, 9.3 h, and 0.4% H2O2 , and enzymatic hydrolysis conditions of 25 FPU cellulase/g treated biomass, 50 oC hydrolysis temperature, 2% biomass loading, and 72 h hydrolysis period, 391.3 mg/g reducing sugar yield was achieved and validated. At the optimized pretreatment and enzymatic conditions, the conversion of treated biomass to untreated biomass was about a 6-fold increase
