Duremdez, Roselyn C.

Lio-Po, Gilda D.

English

Southeast Asian Fisheries Development Center, Aquaculture Department Institutional Repository (SEAFDEC/AQD Institutional Repository )

Southeast Asian Fisheries Development Center Aquaculture DepartmentSEAFDEC/AQD Institutional Repository http://repository.seafdec.org.phJournals/Magazines SEAFDEC Asian Aquaculture1988Isolation and identification ofPseudomonas fluorescens fromhatchery-reared tilapia fry (Oreochromisniloticus Linnaeus)Duremdez, Roselyn C.Aquaculture Department, Southeast Asian Fisheries Development CenterDuremdez, R. C., & Lio-Po, G. D. (1988). Isolation and identification of Pseudomonasfluorescens from hatchery-reared tilapia fry (Oreochromis niloticus Linnaeus). SEAFDEC AsianAquaculture, 10(2), 9.http://hdl.handle.net/10862/2919Downloaded from http://repository.seafdec.org.ph, SEAFDEC/AQD's Institutional RepositoryIsolation and Identification of Pseudomonas fluorescens from Hatchery-Reared Tilapia fry ( Oreochromis niloticus Linnaeus)R.C. Duremdez and G.D. Lio-PoR.C. Duremdez is a Research Associate while G.D. Lio-Po is a Scientist of SEAF­DEC Aquaculture Department.Bacterial infections caused by Pseudomonas sp. are widely report­ed among cultured aquatic organ­isms. In the Philippines, initial report of Pseudomonas sp. in­fections occurred among hatchery- reared tilapia fry, Oreochromis niloticus Linnaeus. Undue stress due to crowding and handling enhanced the development of the disease. The causative agent was identified as Pseudomonas fluo­rescens.Pseudomonas fluorescens is a Gram-negative, rod-shaped bacterial species with flagellar filaments for active motility. It produces green­ish to yellowish diffusable pigmentson selective agar medium such as Pseudosel Agar. Growth and viabi­lity of this bacterium is maintained in optimum freshwater conditions for more than 150 days while via­bility in brackishwater situations is limited to 50 days only. Sea-water medium is not tolerated by this bacterium. This species is thus able to survive and thrive only in freshwater and brackishwater fish culture systems under optimum temperature (25°-30°C).Ps. fluorescens has been fre­quently reported to occur predomi­nantly in the tissues and internal organs of healthy and diseased freshwater fishes such as Oreochro­mis niloticus. The rearing water, likewise, contained predominantly the same genus of the bacteria.Since this bacterial species appears almost always in an aquatic envi­ronment, it is likely to infect the fish under adverse environmental conditions. Undue exposure of the cultured fish to stress brought about by sudden environmental changes and poor rearing conditions such as overcrowding can cause de­terioration of water quality parame­ters. This condition triggers in­creased bacterial load in the water and eventually weaken the fish. Evidence also shows that most outbreaks of infectious diseases are associated with stress. Such situations coupled with the ability of Pseudomonas to thrive longer in an aquatic environment may explain occurrences of Pseudomo­nas infections in aquaculture sys­tems. •_______1987a. An introduction to lapu-lapu (Epinephelus) of the Philip­pines. Part 2 of a series. SEAFDEC Asian Aquacult. 9(1):5, 10._______  1987b. An introduction to lapu-lapu (Epinephelus) of the Philip­pines. Part 3. SEAFDEC Asian Aqua- cult. 9(2):5-8.----------- . 1987c. An introduction to lapu-lapu (Epinephelus) of the Philip­pines. Last of a series. SEAFDEC Asian Aquacult. 9(3):5-8.Manzano VB. 1985. Polyculture system using grouper (Epinephelus tauvina) and tilapia (T. mossambica) in brackishwater pond. R&D J. 2(1):43- 50.Philippines (Republic) Bureau of Fish­eries and Aquatic Resources. Region VIII. 1981. A preliminary report on the observation of groupers (Epine­phelus merra, Epinephelus summana, Cephalopholis patchycentron and Any­perodon leucoaramicus) comparative growth rate of the four species of lapu-lapu culture at Barangay Diit Technical paper, no. 3.Sakares W, Sukbanteang S. 1985. Ex­periment on cage culture of Epinephe­lus tauvina (Forskal) at different den­sity. Proceedings of the 3rd Seminar on Coastal Aquaculture; 1985 May 22-24; Bangkok, Thailand. Bangkok. Brackishwater Fisheries Division, De­partment of Fisheries, Ministry of Agriculture and Cooperatives: 2-29.(In Thai.)Sudradjat A, Oedin H, Amini S. 1985. Effect of feeding methods on the growth of estuarine grouper, Epine­phelus tauvina (Forskal) cultured in floating net-cages. J. Penelitian Budidaya Pantai l(l):45-54. (In Indo­nesian)Teng SK, Chua TE. 1978. Effects of stocking density on the growth of estuary grouper, Epinephelus sal­moides Maxwell, cultured in net- cages. Aquaculture 15:273-287.----------- , ----------- . 1979. Use of arti­ficial hides to increase the stocking density and production of estuary grouper, Epinephelus salmoides Max­well, reared in floating net cages. Aquaculture 16:219-232.SEAFDEC Asian Aquaculture Vol. X No. 2 June 1988 9

Isolation and identification of Pseudomonas fluorescens from hatchery-reared tilapia fry (Oreochromis niloticus Linnaeus)

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Isolation and identification of Pseudomonas fluorescens from hatchery-reared tilapia fry (Oreochromis niloticus Linnaeus)

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