DeLeon, Alyssa

Dhugga, Kanwarpal

Fengler, Kevin

Gaffney, Jim

Jung, Mark

Llaca, Victor

Meeley, Bob

Murithi, Ann

Olsen, Michael

Pixley, Kevin

Schulze, Jan

Simcox, Kevin

English

Purdue E-Pubs

New tools for maize lethal necrosis virus in Africa CIMMYT and Corteva Agrisciencecollaborate on plant breeding innovationsMark Jung, Alyssa DeLeon, Victor Llaca, Kevin Fengler, Bob Meeley, Kevin Simcox, Jan Schulze, Michael Olsen,Ann Murithi, Kanwarpal Dhugga, Kevin Pixley, Jim Gaffneyjim.gaffney@pioneer.comCorteva Agriscience, CIMMYTAbstract: The high-tolerance maize lethal necrosis qualitative trait loci MLN QTL on chr 6L, identified by CIM-MYT, has been narrowed to a small region via positional cloning. Through this collaborative effort involving custommarker development and coordinated cycles of mating and high quality phenotyping, the MLN QTL has been narrowedover 100-fold. Analysis of recombinants continues to further narrow the interval. Full genome optical mapping, se-quencing, and assembly is on-going and analysis will help define a genome editing strategy for CRISPR-edits toprovide MLN tolerance in four CIMMYT elite parent lines, all of which show promising responses in early transfor-mation testing. We will do controlled environment phenotyping to confirm phenotypes in edited materials. The wholegenome sequence approach is now feasible and a valuable tool for exploring genetic diversity, especially for specifictraits e.g. MLN and for informing important tropical and sub-Saharan diversity for future solutions.

New Tools for Maize Lethal Necrosis Virus in Africa:  Cimmyt and Corteva Agriscience Collaborate on Plant Breeding Innovations

https://docs.lib.purdue.edu/cgi/viewcontent.cgi?article=1051&amp;context=scaleup

New Tools for Maize Lethal Necrosis Virus in Africa: Cimmyt and Corteva Agriscience Collaborate on Plant Breeding Innovations

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