Management of prairie dog (Cynomys ludovicianus) movement by colony expansion or dispersal may involve the use of toxicants to reduce local populations. Hazards associated with the use of toxicants cause concern for non-target species. Applying the bait in-burrow should reduce the primary exposure of the toxicants to non-target wildlife. Some literature suggests prairie dogs will not consume bait when applied in the burrow. In this trial we compared efficacy of Rozol® (chlorophacinone), Kaput-D Prairie Dog Bait® (diphacinone), 2% zinc phosphide oats applied in-burrow and 2% zinc phosphide oats applied on the surface. Results are reported as change in prairie dog activity