Electrolyte composition, concentration, pH, ionic strength, and temperature have long been known to play a role in the interface of the electrode-electrolyte. However, detailed studies are not sufficient. Cyclic voltammetry (CV) is a widely used electrochemical technique for obtaining qualitative information in electroanalysis. CV is often the first experiment performed in an electrode surface in an electrochemical study. It measures the number of electrons transferred and intermediates formed during oxidation-reduction (redox) reactions by observing its current, intensity and position of peak potential with applied voltage. This is accomplished by using a three-electrode system including working electrode (WE), counter electrode (CE) and reference electrode (RE). In this work, our system consists of gold (Au) We, platinum (PT) CE and silver/silver chloride (Ag/AgCl) RE. The potential relative to the reference electrode is forward- and reverse-scanned at the working electrode while the current flows through the counter electrode is observed in a solution. In this study, we investigate systematically the redox potential of the different analytes under various conditions in concentration and pH. Therefore, this study can be referenced for studies in exploring a variety range of biological samples, cells, tissues, lipids, and proteins on surfaces
