Raw research data supporting the publication:

Armstron, JPK et al., "Engineering anisotropic muscle tissue using acoustic cell paterning", Advanced Materials, DOI: 10.1002/adma.201802649 (2018

Armstrong, JPK

Assal, V

Bassindale, PG

Breant, A

Drinkwater, BW

Franklin, AC

Guex, AG

Hutmacher, DW

Kapnisi, M

King, O

Klein, TJ

Meinert, C

Murty, T

Nichols, MK

Perriman, AW

Puetzer, JL

Serio, A

Skaalure, SC

Stevens, MM

Terracciano, CM

Zong, Y

Advanced Materials

Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics

Armstrong, J

Kapnisi, K

Skaalure, S

Terracciano, C

Spiral - Imperial College Digital Repository

English

Engineering anisotropic muscle tissue using acoustic cell patterning

ZENODO

Research data supporting "Engineering anisotropic muscle tissue using acoustic cell patterning"

NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY

Tissue engineering has offered unique opportunities for disease modelling and regenerative medicine, however, the success of these strategies is dependent upon faithful reproduction of native cellular organization. Here, we report that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibited significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic pattering of myoblasts in gelatin methacryloyl hydrogels significantly enhanced myofibrillogenesis and promoted the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120-150 μm and a spacing of 180-220 μm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically-patterned cells. We anticipate that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially-organized cell cultures, organoid development and bioelectronics

Armstrong, James P.K.

Puetzer, Jennifer L.

Serio, Andrea

Guex, Anne Géraldine

Kapnisi, Michaella

Breant, Alexandre

Zong, Yifan

Assal, Valentine

Skaalure, Stacey C.

King, Oisín

Murty, Tara

Meinert, Christoph

Franklin, Amanda C.

Bassindale, Philip G.

Nichols, Madeleine K.

Terracciano, Cesare M.

Hutmacher, Dietmar W.

Drinkwater, Bruce W.

Klein, Travis J.

Perriman, Adam W.

Stevens, Molly M.

Explore Bristol Research

Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

Raw research data supporting the publication:

Armstron, JPK et al., "Engineering anisotropic muscle tissue using acoustic cell paterning", Advanced Materials, DOI: 10.1002/adma.201802649 (2018)</p

Armstrong, JPK (5791985)

Puetzer, JL (5791988)

Serio, A (5791991)

Guex, AG (5791994)

Kapnisi, M (5791997)

Breant, A (5792000)

Zong, Y (5792003)

Assal, V (5792006)

Skaalure, SC (5792009)

King, O (5792012)

Murty, T (5792015)

Meinert, C (5792018)

Franklin, AC (5792021)

Bassindale, PG (5792024)

Nichols, MK (5792027)

Terracciano, CM (5792030)

Hutmacher, DW (5792033)

Drinkwater, BW (5792036)

Klein, TJ (5792039)

Perriman, AW (5792042)

Stevens, MM (5177570)

The Francis Crick Institute

Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.Full Tex

Armstrong, James PK

Puetzer, Jennifer L

Guex, Anne Geraldine

Skaalure, Stacey C

King, Oisin

Franklin, Amanda C

Bassindale, Philip G

Nichols, Madeleine K

Terracciano, Cesare M

Hutmacher, Dietmar W

Drinkwater, Bruce W

Klein, Travis J

Perriman, Adam W

Stevens, Molly M

Griffith Research Online

Armstrong, James

Puetzer, Jennifer

Guex, A

Skaalure, Stacey

Franklin, A

Bassindale, P

Nichols, M

Hutmacher, D

Drinkwater, B

Klein, T

Perriman, A

Stevens, M

http://spiral.imperial.ac.uk/bitstream/10044/1/63350/9/Armstrong_et_al-2018-Advanced_Materials.pdf

Research data supporting "Engineering anisotropic muscle tissue using acoustic cell patterning"

Abstract

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Spiral - Imperial College Digital Repository

ZENODO

NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY

Explore Bristol Research

The Francis Crick Institute

Griffith Research Online

Spiral - Imperial College Digital Repository