Bannister, I

Daniels, S

Moore-Colyer, M

Daniels, Simon

Moore-Colyer, Meriel

Royal Agricultural University Repository

The effect of a concentrate based diet (CD) or a total mixed fibre ration (TMFR) on in vitro stomach and hindgut health   Isobel Bannister 1,2, Simon Daniels 1  and MJS Moore-Colyer1 1 Royal Agricultural University, Cirencester Glos. GL7 6JS UK 2 Hartpury University Centre, Hartpury, Glos. GL19 3BE UK  Background: Maximum growth rates in young stock are often achieved by feeding high-levels of cereal based concentrates. This can negatively impact digestive health and alter the composition of hindgut microbiota.  Objective: Determine the effect of TMFR versus CD on simulated stomach and hindgut pH and hindgut d-Lactate concentrations. Method: Stomach digestion was simulated in vitro in a shaking incubator, pH loggers (Neulog Edu-logger 900-209) recorded pH at 30 second intervals over 6 hours. Feed (10g) and saliva (NaCl 2.9g/L, KHCO3 1.5g/L and NaHCO3 2.8g/L) were added to pH 6.0 HCL incubated at 37°C. After 2 hours pH was reduced to pH 5.4 at 4 hours pH was adjusted to 2.6, pepsin (0.4g) added and incubated for a final 2 hours.  Faeces from foals fed TMFR (n=4) or concentrates (n=4) were used as microbial inocula for in vitro gas production. Post inoculation bottles were harvested at 4, 12, 24, 62 hours for pH and d-lactate. D-lactate was measured via colourmetric assay (Sigma-Aldrich). Data were analysed by ANOVA and Fisher least significant difference (LSD) test post hoc. Results: Stomach pH was lower for the CD than TMFR in each simulated region (P<0.001). Hindgut pH at 12 hrs was higher in CD than TMFR whereas the opposite occurred at 24 hours. D-lactate was higher (x2) in CD fermentations (P=0.023), at both 12 and 24 hours.  Conclusion: In vitro TMFR kept stomach and hindgut pH and hindgut d-lactate closer to optimum compared to CD reducing potential risks to digestive health in young stock.   

The effect of a concentrate based diet (CD) or a total mixed fibre ration (TMFR) on in vitro stomach and hindgut digestion and gut health

https://rau.repository.guildhe.ac.uk/id/eprint/16356/1/EWEN%20TMFR%20in%20vitro%20.pdf

The effect of a concentrate based diet (CD) or a total mixed fibre ration (TMFR) on in vitro stomach and hindgut digestion and gut health

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