The effect of light to moderate intensity exercise, such as that used as a mode of transport, on glycaemic response (GR) testing is unclear. The aim was to investigate the effect of acute exercise (walking and cycling) simulated to act as a mode of transport, prior to GR testing on the intra-individual variability of blood glucose and insulin. Eleven male participants visited the laboratory four times. Initially they undertook a VO2max and two submaximal exercise tests. For the other three visits they either rested (25 min), cycled or walked 5km followed by a two hour GR test after consuming a glucose drink (50g available carbohydrate). The mean CV of each transport group was below the International Organisation for Standardisation cut off of 30%. The highest mean coefficient of variation (CV) of glucose area under the curve (GAUC) was between the rest and walking trials (30%) followed by walking and cycling (26%). For insulin AUC (IAUC) the highest mean CV was between walking and cycling (28%) followed by rest and walking (24%). The lowest GAUC and IAUC were between rest and cycling (25% and 14%, respectively). The current study also did not find differences (p >.05) between the conditions for GAUC (rest: 134.5 ± 104.6; walking: 115.5 ± 71.7; cycling: 142.5 ± 75 mmol·120min·L-1) and IAUC (rest: 19.45 ± 9.12; walking: 16.49 ± 8.42; cycling: 18.55 ± 9.23 µmol·120min·mL-1). The results indicate no difference between the tests undertaken however further research should ensure the inclusion of two rest conditions
