Crooke, H

Crudgington, B

Everett, H

Skinner, M

Cytokine

English

Bentley Crudgington

Helen Everett

Mike Skinner

Helen Crooke

Crossref

ID: 233

Crudgington, Bentley

Everett, Helen

Skinner, Mike

Crooke, Helen

Spiral - Imperial College Digital Repository

Investigation	of	Porcine	Interferons	as	a	metaphylactic	intervention	strategy	against	Classical	Swine	Fever	Virus	(CSFV)		Bentley	Crudgington1*,	Helen	Everett1,	Mike	Skinner2,	Helen	Crooke1			1	APHA,	United	Kingdom		2	Imperial	College	London,	United	Kingdom		*	Corresponding	author.		Classical	Swine	Fever	(CSF)	is	an	important	viral	disease	of	swine.	The	application	of	Interferon	(IFN)	via	viral	vectors	could	be	a	beneficial	intervention	strategy	against	CSFV.	Unlike	type	I	and	II	IFN,	the	anti-CSFV	activity	of	type	III	IFNs	are	unknown.	Type	III	IFNs	have	similar	antiviral	actions	to	type	I	IFN	but	their	specific	receptors	have	limited	tissue	distribution	and	may	be	important	as	very	early	defences	against	infection	at	epithelial	surfaces.	PoIFN	(type	I	(α;	β)	type	II	(γ)	and	type	III	(λ1,	3))	were	cloned	into	a	mammalian	expression	vector,	expressed	in	porcine	tracheal	(NPTR)	cells	and	the	induction	of	an	immune	response	and	anti-CSFV	activity	examined.	PoIFNs	were	subsequently	cloned	into	an	Adenovirus	viral	vector	and	the	antiviral	activity	assessed.	All	three	types	of	IFN	upregulated	expression	of	the	interferon	stimulated	gene	MxA	and	significantly	reduced	CSFV	infection	in	the	NPTR	cell	line	and	in	porcine	primary	cells.	Inoculation	of	NPTR	cells	with	adenovirus	vectors	expressing	all	three	types	of	IFN	inhibited	CSFV	infection,	with	the	AdIFNα/β	and	λ	constructs	induc-	ing	a	greater	anti-CSFV	effect	than	the	AdIFNγ	constructs.		Conclusion:	All	three	types	of	porcine	IFN	have	some	antiviral	activity	against	CSFV	and	delivery	via	adenovirus	vectors	may	offer	a	promising	targeted	intervention	strategy	against	CSF.	Future	studies	will	assess	the	kinetics	of	protection	of	single	and	combined	vectored	PoIFN	treatments	in	vitro	and	in	vivo.	

Investigation of Porcine Interferons as a metaphylactic intervention strategy against Classical Swine Fever Virus (CSFV)

http://spiral.imperial.ac.uk/bitstream/10044/1/57760/2/Bentley%20Crudgington%20Bamberg%20Cytokines%202015%20poster.pdf

Investigation of Porcine Interferons as a metaphylactic intervention strategy against Classical Swine Fever Virus (CSFV)

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