Ormond, E. L.

Pell, J. K.

Roy, H. E.

Thomas, A. P. M.

English

NERC Open Research Archive

Molecular characterisation of Beauveria bassiana isolates obtained from semi-field arenas of overwintering Coccinella septempunctata   Isolates of Beauveria bassiana were obtained from Coccinella septempunctata overwintering in semi-field arenas at the Genetics Research Center in Cambridge, UK. Beauveria bassiana was also isolated from the semi-field arena soil by Galleria melonella baiting and serial dilution plating on selective media. DNA was extracted from these isolates using ISSR-PCR, and four ISSR primers were used to characterise and to investigate genetic diversity of the isolates at the molecular level. The ISSR-PCR detected a high level of genetic variation among the isolates. Analyses of this data gave indications of intra-specific groupings correlated with source of origin. The grouping of isolates by source of origin, perhaps suggests the overwintering Coccinella septempunctata populations were infected by a sub-section of the native population with superior entomopathogenic abilities. 

Molecular characterisation of Beauveria bassiana isolates obtained from semi-field arenas of overwintering Coccinella septempunctata

http://nora.nerc.ac.uk/1150/1/OrmondMolecular.pdf

Molecular characterisation of Beauveria bassiana isolates obtained from semi-field arenas of overwintering Coccinella septempunctata

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