Bailey, Sarah

Mackenzie, Amanda

Ver Donck, Luc

Wickens, Robin

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English

The effect of housing conditions on lipopolysaccharide-induced depressive-like behaviour in mice

        Citation for published version:Wickens, R, Ver Donck, L, Mackenzie, A & Bailey, S 2015, 'The effect of housing conditions onlipopolysaccharide-induced depressive-like behaviour in mice', Journal of Psychopharmacology , vol. 29, no. 8(Abstract Supplement), A08, pp. A17.Publication date:2015Document VersionPeer reviewed versionLink to publicationThis is the author's accepted version of a conference abstract, published by Sage Publications in final form at:http://www.bap.org.uk/pdfs/2015BAPAbstractBook.pdfUniversity of BathGeneral rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Download date: 13. May. 2019The effect of housing conditions on lipopolysaccharide-induced depressive-like behaviour in mice Robin A. Wickens, Luc Ver Donck1, Amanda B. Mackenzie & Sarah J. Bailey Pharmacy & Pharmacology, University of Bath 1Department of Neuroscience, Janssen Research & Development Inflammation is thought to play a key role in the pathology of depression. Acute administration of lipopolysaccharide (LPS) is often used to assess the role of inflammation in depressive-like behaviours, such as the forced swim test (FST), in mice (O’Connor et al. 2009. Mol. Psychiatry. 14(5), 511-522). We have previously shown that acute LPS failed to induce a depressive-like behaviour in the FST (Wickens et al. 2014. J. Phsychopharmacol. 28(8), A107). Baseline depressive-like behaviour is influenced by environmental factors, including lighting and housing conditions (Bogdanova et al. 2013. Physiol. Behav. 118, 227-239). Here, we have investigated the influence of the light cycle and group/individual housing on LPS-induced depressive-like behaviour in mice. Adult male C57BL/6J mice (10-14 weeks, Charles River) were randomly assigned to LPS or control groups (n=9-15/group). Mice were housed individually or in groups of 3-4 under a normal (lights on: 06:00) or reverse (lights on: 18:00) 12-hour light cycle. LPS (0.415 or 0.83mg/kg, i.p.) or saline was injected before being tested in the open field test (OFT) at +6 or +24 hours to assess sickness and the FST at +24 hours to assess depressive-like behaviour. Immobility in the FST was manually scored for 6 minutes. Distance travelled in the OFT was analyzed using automated video analysis. Data were analysed using one-way ANOVA followed by Dunnett posthoc test. Reduced locomotion was seen 6 hours after LPS administration in all housing conditions, including ‘group-housed/light-phase’ (F(2,33)=49.82, P<0.001), ‘individual-housed/light-phase’ (F(2,27)=70.48, P<0.001), ‘group-housed/dark-phase’ (F(2,40)=78.56, P<0.001) and ‘individual-housed/dark-phase’ (F(2,33)=97.65, P<0.001). LPS had a significant effect on immobility 24 hours after LPS administration in ‘group-housed/light-phase’ mice (F(2,29)=5.582, P<0.0089), with 0.415mg/kg LPS inducing a significant increase in immobility (P<0.05), but had no effect in all other housing and lighting conditions. Reduced locomotion was still present in the OFT at 24 hours after LPS (F(2,49)=8.621, P<0.001). Whilst sickness behaviour, as interpreted by reduced locomotion in the OFT, was observed in all LPS-treated groups, depressive-like behaviour in the FST was only observed in one group: group-housed/light-phase, 0.415mg/kg LPS. This finding suggests that the depressive response to LPS in the FST is sensitive to the housing conditions, along with the dose of LPS used. However, reduced locomotion was observed at 24 hours, indicating the presence of sickness, which is a confounding factor for depressive-like behaviour in the FST observed at 24 hours. Support by MRC CASE PhD studentship with Janssen Research and Development, a Division of Janssen Pharmaceutica NV 

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The effect of housing conditions on lipopolysaccharide-induced depressive-like behaviour in mice

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