We are grateful to the Microscopy and Histology Core Facility of the University of Aberdeen for their assistance with sample processing. This work was generously funded by the charity Saving Sight in Grampian of the University of Aberdeen Development Trust.Peer reviewedPublisher PD

Forrester, John V. Vincent

Kuffova, Lucia

Liu, Yi-Hsia

Milne, Gillian C.

Moelzer, Christine

Data in Brief

English

Histology (H&E) and transmission electron microscopy (TEM) data are provided showing age-related changes in the retinal structure of sTg-IRBP:HEL mice. These include substantial photoreceptor loss, atrophy of the retinal pigment epithelium, Bruch׳s membrane disruption and thickening, along with the presence of drusenoid deposits and changes in basal laminar infoldings. These features resemble some of those key characteristics found in the course of human dry (atrophic) age-related macular degeneration (AMD), particularly with regard to drusen. Hence, we believe the sTg-IRBP:HEL mouse model represents a useful and promising archetype for future study of the mechanism of drusen formation in AMD

Yi-Hsia Liu

Christine Mölzer

Gillian C. Milne

Lucia Kuffová

John V. Forrester

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Transmission Electron Microscopy Data on drusen-like deposits in the retinal degeneration sTg-IRBP: HEL mouse model

We are grateful to the Microscopy and Histology Core Facility of the University of Aberdeen for their assistance with sample processing. This work was generously funded by the charity Saving Sight in Grampian of the University of Aberdeen Development Trust.Peer reviewe

Aberdeen University Research

Contents lists available at ScienceDirectData in BriefData in Brief 22 (2019) 140–144https://2352-34(http://cn CorrE-mgillian.mjournal homepage: www.elsevier.com/locate/dibData ArticleTransmission Electron Microscopy Dataon drusen-like deposits in the retinal degenera-tion sTg-IRBP: HEL mouse modelYi-Hsia Liu a, Christine Mölzer a, Gillian C. Milne b,Lucia Kuffová a,c, John V. Forrester a,d,na Institute of Medical Sciences, University of Aberdeen, Aberdeen, UKb Microscopy and Histology Core Facility, University of Aberdeen, Aberdeen, UKc Department of Ophthalmology, NHS Grampian, Aberdeen, UKd University of Western Australia, Lions Eye Institute, Perth, Australiaa r t i c l e i n f oArticle history:Received 22 October 2018Received in revised form20 November 2018Accepted 3 December 2018Available online 6 December 2018doi.org/10.1016/j.dib.2018.12.00709/& 2018 The Authors. Published by Elsereativecommons.org/licenses/by/4.0/).espondence author.ail addresses: yi-hsia.liu@abdn.ac.uk (Y.-H.ilne@abdn.ac.uk (G.C. Milne), l.kuffova@aba b s t r a c tHistology (H&E) and transmission electron microscopy (TEM) dataare provided showing age-related changes in the retinal structureof sTg-IRBP:HEL mice. These include substantial photoreceptorloss, atrophy of the retinal pigment epithelium, Bruch's membranedisruption and thickening, along with the presence of drusenoiddeposits and changes in basal laminar infoldings. These featuresresemble some of those key characteristics found in the course ofhuman dry (atrophic) age-related macular degeneration (AMD),particularly with regard to drusen. Hence, we believe the sTg-IRBP:HEL mouse model represents a useful and promising archetype forfuture study of the mechanism of drusen formation in AMD.& 2018 The Authors. Published by Elsevier Inc. This is an openaccess article under the CC BY license(http://creativecommons.org/licenses/by/4.0/).vier Inc. This is an open access article under the CC BY licenseLiu), christine.moelzer@abdn.ac.uk (C. Mölzer),dn.ac.uk (L. Kuffová), j.forrester@abdn.ac.uk (J.V. Forrester).Y.-H. Liu et al. / Data in Brief 22 (2019) 140–144 141Speciﬁcations tableSMTHDEEDDRubject area Medicine and Dentistryore speciﬁc subject area Ophthalmologyype of data Transmission electron microscopy (TEM) and histology (H&E) images.ow data was acquired Light microscope: Carl Zeiss Axioskop 40 with ProgRes XT Core 5 colourdigital microscope camera.Transmission electron microscope: JEOL 1400 plus; AMT UltraVUE camera.ata format Acquired and analysed.xperimental factors Eyes and retinas collected from B10.BR and sTg-IRBP:HEL mice.xperimental features TEM shows age-related retinal degeneration in sTg-IRBP:HEL mice.ata source location University of Aberdeen, Institute of Medical Sciences, Foresterhill, AB252ZD, Aberdeen, UK (57.156669, -2.135100).ata accessibility The data will be available with this article.elated research article Liu Y-H, Corbett C, Klaska IP, Makinen K, Nickerson JM, Cornall RJ, KuffovaL, Forrester JV. Partial Retinal Photoreceptor Loss in Transgenic MouseDue to Reduced Levels of Interphotoreceptor Retinol Binding Protein(IRBP, RBP3). Experimental eye research 172, 54–65. [1]Value of the data Despite being a leading cause of blindness in the UK, resulting in a gradual loss of (central) vision, aswell as an economic burden, the underlying pathogenesis of especially dry AMD is not understood. The atrophic (dry) form of the disease is more prevalent, and there is no treatment available at thepresent time. The data in this DIB report offer a new model for dry AMD which will allowresearching of therapeutic options. In contrast to their WT controls, sTg-IRBP:HEL mice exhibit age-related drusenoid deposits,resembling those seen in human dry AMD. This animal model is compatible with the clinical cardinal features of human dry AMD and mayprove beneﬁcial for future mechanistic AMD research and therapy.1. DataSingle transgenic IRBP:HEL mice expressing hen egg lysozyme (HEL) under the retinal inter-photoreceptor retinoid-binding protein (IRBP, RBP3) promoter, were generated as previously reported[2]. With age, these mice gradually lose the expression of interphotoreceptor retinoid-binding protein(IRBP; RBP3). In our experiments, we compared 60 day old (“adult”) or 240–300 day old (“aged”) wildtype mice (WT; P60 and P240–300, respectively), with said transgenic animals of the same age groupsfor the cardinal features resembling human atrophic (dry) AMD. Central retinal evaluation by TEMrevealed absence of age-related changes occurring in retinas of control adult (P60) WT mice (Fig. 1A-C), while in sTg-IRBP:HEL mice of the same age (Fig. 1D-F) drusen-like deposits were found thataccumulated with age (Fig. 1F). These adult mice showed signs of slightly disorganized/shortenedphotoreceptor layers (Fig. 1D, E) and Bruch's membrane thickening with extensive alteration in RPEbasal infoldings (Fig. 1F, circle). In terms of retinal degenerative markers, aged control mice (P240–300, WT; Fig. 1G-I) were comparable to adult (P60) sTg-IRBP:Hel mice. In aged sTg-IRBP:HEL mice(P240–300) retinal degeneration became increasingly evident, with complete loss of photoreceptors(Fig. 1J, K) and large sub-RPE drusen-like deposits detected (Fig. 1L). These degenerative features arein agreement with those encountered in human atrophic AMD, thus we propose the sTg-IRBP:HELmouse model as an accessible and useful vehicle for future AMD research. Data are summarised anddescribed in Table 1, statistics are provided in Table 2.Fig. 1. shows representative central retinal sections of 60 day old (P60; “adult”) WT mice (A-C), vs. their P60 sTg-IRBP:HELcounterparts (D-F), and of “aged” (P240–300) WT mice (G-I) vs. P240–300 sTg-IRBP:HEL mice (J-L). H&E histology and TEMpictures are provided along with medians of n ¼ 3 independent measurements per marker. In the adult WT mice, all retinallayers appear complete and intact (A), photoreceptors (180.6 mm) and RPE are well preserved (5.2 mm) (B), and the Bruch'smembrane shows normal occurrence and thickness (376.3 nm) (C). In the sTg-IRBP:HEL P60 mice, the photoreceptors arepartially lost (66.0 mm; D, E), together with BM thickening (1075.0 nm) and abundant drusenoid deposits (F; circle). Comparableto the adult sTg-IRBP:Hel mice, in the aged WT mice (G-I), all retinal layers appear intact, with photoreceptors (118.1 mm) andRPE (4.3 mm) slightly diminished but preserved (G, H). In the aged sTg-IRBP:HEL mice, however, the photoreceptors arecompletely lost (10.4 mm; J), retinal pigment epithelium further reduced (2.6 mm), and BM thickening and drusenoid depositsare increasingly seen (806.5 nm; K, L; circle). Circle: BM membrane disruption with drusenoid deposit and disorganised basallaminar infoldings; Ch: charcoal-like granule; R: RPE atrophy/loss of pigment; P: undigested POS phagosome; L: lipofuscingranule; Cy: cystic membranous degradation of RPE. Evaluation of retinas for AMD-like features (i.e. BM disruption, drusenoiddeposits, loss of RPE pigment, lipofuscin deposition, charcoal-like granules, and undigested POS) followed recent reports byPark et al. [3], and Ramkumar et al. [4]. Abbreviations: RGL, retinal ganglion layer; IPL, inner plexiform layer; INL, inner nuclearlayer; OPL, outer plexiform layer; ONL, outer nuclear layer; PIS, photoreceptor inner segments; POS, photoreceptor outer seg-ments; BM, Bruch's membrane; CH, choroid.Y.-H. Liu et al. / Data in Brief 22 (2019) 140–1441422. Experimental design, materials, and methods2.1. AnimalsThe generation of sTg-IRBP:HEL mice was previously described [2]. All procedures performed werein agreement with the regulations of the Animal License Act (UK) and followed the ARRIVE guidelinesfor animal husbandry. All mice were bred in established breeding colonies and maintained/housed inthe Medical Research Facility of the University of Aberdeen. Mice genotypes were veriﬁed by a rou-tinely used in-house PCR protocol.Table 2compares mice groups based on age (P60 vs. P240-300: post-partum day 60, "adult" vs. day 240-300, "old"), or genotype (WT:wildtype vs. sTg-IRBP:HEL: single transgenic). P-values are provided; asterisks denote signiﬁcant differences based on a Z 95%level of conﬁdence. Medians (50th percentile) of n ¼ 3 independent measurements per marker of interest were compared usingthe Mann-Whitney U-test.PIS/POS,p-ValueRPE, p-Value BM, p-ValueWT P60 (“adult”) 0.046* 0.043* 0.127WT P240–300 (“old”)sTg-IRBP:HEL P60 (“adult”) 0.046* 0.050* 0.050*sTg-IRBP:HEL P240–300 (“old”)WT P60 (“adult”) 0.050* 0.046* 0.050*sTg-IRBP:HEL P60 (“adult”)WT P240–300 (“old”) 0.043* 0.178 0.513sTg-IRBP:HEL P240–300 (“old”)Table 1speciﬁes photoreceptor inner- (PIS) and outer layer (POS), retinal pigment epithelium (RPE), and Bruch's Membrane (BM)thickness as presented in Fig. 1. Medians (50th percentile) and ranges (in brackets) of n ¼ 3 measurements each are provided.Wild type (WT; controls) and IRBP:HEL single transgenic (sTg) mice of two age groups were used (P60: post-partum day 60,“adult”; P240–300: post-partum day 240–300, “old”).PIS/POS [lm] RPE [lm] BM [nm]WT P60 (“adult”) 180.6 (20.8) 5.2 (0.3) 376.3 (172.0)WT P240–300 (“old”) 118.1 (7.0) 4.3 (1.7) 537.6 (215.1)sTg-IRBP:HEL P60 (“adult”) 66.0 (6.9) 3.9 (1.0) 1075.0 (268.8)sTg-IRBP:HEL P240–300 (“old”) 10.4 (3.5) 2.6 (0.7) 806.5 (451.7)Y.-H. Liu et al. / Data in Brief 22 (2019) 140–144 1432.2. Sample preparation for histology, and H&E stainingWild type mice (B10.BR) and sTg-IRBP:HEL mice of different ages (P60 and P240–300, respectively)were humanely killed and eyes removed immediately. Per age- and test group 2 to 4 animals wereanalysed. Eyes were ﬁxed in 2.5% (w/v) glutaraldehyde (Fisher Chemicals, Loughborough, UK) andembedded in resin to be sectioned for standard H&E staining. Images were recorded using a ProgResXT Core 5 colour digital microscope camera (JENOPTIK Optical Systems GmbH, Jena, Germany) withsamples mounted on an inverted microscope (Axioskop40, Carl Zeiss, MicroImaging GmbH, Jena,Germany).2.3. Sample preparation for TEMAs above, mouse eyes (WT and sTg-IRBP:HEL; P60 and P240–300, respectively) were collected andﬁxed in 2.5% glutaraldehyde. Anterior segments (i.e. cornea, sclera, iris and lens) were removed. Theremaining eyecup (vitreous) was then post-ﬁxed in osmium tetroxide, dehydrated in ethanol, fol-lowed by acetone, and embedded in Spurr's resin. Ultrathin sections were cut using a Leica UC6microtome (Leica Microsystems), stained with uranyl acetate and lead citrate, and examined with aTEM microscope (JEOL 1400 plus, equipped with an AMT UltraVUE camera).2.4. Statistical analysisStatistics were performed using IBM SPSS Statistics 25.0. Based on the nature of the data available,the non-parametric procedure of Mann-Whitney U-test was used to compare data based on ranks.Y.-H. Liu et al. / Data in Brief 22 (2019) 140–144144Medians (50th percentile) and ranges are presented in Table 1, along with p-values based on a Z 95%level of conﬁdence in Table 2.AcknowledgmentsWe are grateful to the Microscopy and Histology Core Facility of the University of Aberdeen fortheir assistance with sample processing. This work was generously funded by the charity Saving Sightin Grampian of the University of Aberdeen Development Trust (UK) [grant No. RG 14058-10].Transparency document. Supporting informationTransparency data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2018.12.007.References[1] Y.-H. Liu, et al., Partial retinal photoreceptor loss in a transgenic mouse model associated with reduced levels of inter-photoreceptor retinol binding protein (IRBP, RBP3), Exp. Eye Res. 172 (2018) 54–65.[2] T. Lambe, et al., Limited peripheral T cell anergy predisposes to retinal autoimmunity, J. Immunol. 178 (7) (2007) 4276.[3] S.W. Park, et al., Dry age-related macular degeneration like pathology in aged 5XFAD mice: ultrastructure and microarrayanalysis, Oncotarget 8 (25) (2017) 40006–40018.[4] H.L. Ramkumar, J. Zhang, C.-C. Chan, Retinal ultrastructure of murine models of dry age-related macular degeneration(AMD), Prog. Retin. Eye Res. 29 (3) (2010) 169–190.

Transmission Electron Microscopy Data on drusen-like deposits in the retinal degeneration sTg-IRBP : HEL mouse model

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Transmission Electron Microscopy Data on drusen-like deposits in the retinal degeneration sTg-IRBP : HEL mouse model

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