Botsios, Sotirios

Donaldson, Anne Dunlop

Donze, David

Hiraga, Shinichiro

Molecular Biology of the Cell

English

PubMed

Chromatin function requires specific three-dimensional architectures of chromosomes. We investigated whether Saccharomyces cerevisiae extra TFIIIC (ETC) sites, which bind the TFIIIC transcription factor but do not recruit RNA polymerase III, show specific intranuclear positioning. We show that six of the eight known S. cerevisiae ETC sites localize predominantly at the nuclear periphery, and that ETC sites retain their tethering function when moved to a new chromosomal location. Several lines of evidence indicate that TFIIIC is central to the ETC peripheral localization mechanism. Mutating or deleting the TFIIIC-binding consensus ablated ETC -site peripheral positioning, and inducing degradation of the TFIIIC subunit Tfc3 led to rapid release of an ETC site from the nuclear periphery. We find, moreover, that anchoring one TFIIIC subunit at an ectopic chromosomal site causes recruitment of others and drives peripheral tethering. Localization of ETC sites at the nuclear periphery also requires Mps3, a Sad1-UNC-84-domain protein that spans the inner nuclear membrane. Surprisingly, we find that the chromatin barrier and insulator functions of an ETC site do not depend on correct peripheral localization. In summary, TFIIIC and Mps3 together direct the intranuclear positioning of a new class of S. cerevisiae genomic loci positioned at the nuclear periphery. © 2012 Hiraga et al

Hiraga, Shin Ichiro

Donaldson, Anne D.

LSU Scholarly Repository (Louisiana State Univ.)

TFIIIC localizes budding yeast ETC sites to the nuclear periphery

Aberdeen University Research

A CBF5 mutation that disrupts nucleolar localization of early tRNA biosynthesis in yeast also suppresses tRNA gene-mediated transcriptional silencing.

A comprehensive genomic binding map of gene and chromatin regulatory proteins in Saccharomyces.

A new efficient gene disruption cassette for repeated use in budding yeast.

A role for TFIIIC transcription factor complex in genome organization.

a stable replication-pausing complex.

A three-dimensional model of the yeast genome.

A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.

An auxin-based degron system for the rapid depletion of proteins in nonplant cells.

Architectural organization in the interphase nucleus of the protozoan Trypanosoma brucei: location of telomeres and mini-chromosomes.

Autoregulation of an RNA polymerase II promoter by the RNA polymerase III transcription factor III C (TF(III)C) complex.

Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

Centromere position in budding yeast: evidence for anaphase A.

Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes.

Cohesins functionally associate with CTCF on mammalian chromosome arms.

Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.

Developmentally induced changes in transcriptional program alter spatial organization across chromosomes.

Differences in the localization and morphology of chromosomes in the human nucleus.

Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells.

Early initiation of a replication origin tethered at the nuclear periphery.

Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C.

European Functional Analysis Network (EUROFAN) and the functional analysis of the Saccharomyces cerevisiae genome.

Geiduschek EP

Gene recruitment of the activated INO1 locus to the nuclear membrane.

Genome-wide location of yeast RNA polymerase III transcription machinery.

Genome-wide occupancy profile of the RNA polymerase III machinery in Saccharomyces cerevisiae reveals loci with incomplete transcription complexes.

Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells.

Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells.

Global analysis of protein expression in yeast.

Histone H3 lysine 56 acetylation by Rtt109 is crucial for chromosome positioning.

Human telomeres are attached to the nuclear matrix.

Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.

In vivo localization of DNA sequences and visualization of largescale chromatin organization using lac operator/repressor recognition.

Insulators: many functions, many mechanisms.

Ku-deficient yeast strains exhibit alternative states of silencing competence.

Live imaging of telomeres: yKu and Sir proteins define redundant telomereanchoring pathways in yeast.

Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast.

Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery.

Meiotic chromosome pairing in maize is associated with a novel chromatin organization.

Multiple bromodomain genes are involved in restricting the spread of heterochromatic silencing at the Saccharomyces cerevisiae HMR-tRNA boundary.

Multiple pathways for telomere tethering: functional implications of subnuclear position for heterochromatin formation.

Nonrandom radial arrangements of interphase chromosome territories: evolutionary considerations and functional implications.

Novel chromophores and buried charges control color in mFruits.

Nucleolar clustering of dispersed tRNA genes.

Nucleolar localization of early tRNA processing.

Nucleolar organizers in human oocytes at meiotic prophase I, studied by the silver-NOR method and electron microscopy.

Nucleoporin mediated nuclear positioning and silencing of HMR.

Nup-PI: the nucleopore-promoter interaction of genes in yeast.

Ordered nucleation and spreading of silenced chromatin in Saccharomyces cerevisiae.

Pagano A

Perinuclear localization of chromatin facilitates transcriptional silencing.

Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.

Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli.

Putting boundaries on silence.

Recruitment to the nuclear periphery can alter expression of genes in human cells.

Release of yeast telomeres from the nuclear periphery is triggered by replication and maintained by suppression of Ku-mediated anchoring.

Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site.

S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors.

Sentenac A

Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.

Spatial genome organization during T-cell differentiation.

Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z.

Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.

TFIIIC binding sites function as both heterochromatin barriers and chromatin insulators in Saccharomyces cerevisiae.

The clustering of telomeres and colocalization with

The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time.

The nuclear envelope—a scaffold for silencing?

The positioning and dynamics of origins of replication in the budding yeast nucleus.

The RNA polymerase III transcriptome revealed by genome-wide localization and activity-occupancy relationships.

The subunit structure of Saccharomyces cerevisiae transcription factor IIIC probed with a novel photocrosslinking reagent.

The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae.

Tissue-specific spatial organization of genomes.

Transcription independent insulation at TFIIIC-dependent insulators.

Translational and rotational settings of H2A.Z nucleosomes across the Saccharomyces cerevisiae genome.

tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter.

Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.

We gather together: insulators and genome organization.

Yeast nuclei display prominent centromere clustering that is reduced in nondividing cells and in meiotic prophase.

Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombination.

TFIIIC Localizes Budding Yeast ETC Sites to the Nuclear Periphery

Chromatin function requires specific three-dimensional architectures of chromosomes. We investigated whether Saccharomyces cerevisiae extra TFIIIC (ETC) sites, which bind the TFIIIC transcription factor but do not recruit RNA polymerase III, show specific intranuclear positioning. We show that six of the eight known S. cerevisiae ETC sites localize predominantly at the nuclear periphery, and that ETC sites retain their tethering function when moved to a new chromosomal location. Several lines of evidence indicate that TFIIIC is central to the ETC peripheral localization mechanism. Mutating or deleting the TFIIIC-binding consensus ablated ETC -site peripheral positioning, and inducing degradation of the TFIIIC subunit Tfc3 led to rapid release of an ETC site from the nuclear periphery. We find, moreover, that anchoring one TFIIIC subunit at an ectopic chromosomal site causes recruitment of others and drives peripheral tethering. Localization of ETC sites at the nuclear periphery also requires Mps3, a Sad1-UNC-84–domain protein that spans the inner nuclear membrane. Surprisingly, we find that the chromatin barrier and insulator functions of an ETC site do not depend on correct peripheral localization. In summary, TFIIIC and Mps3 together direct the intranuclear positioning of a new class of S. cerevisiae genomic loci positioned at the nuclear periphery.</jats:p

Shin-ichiro Hiraga

Sotirios Botsios

David Donze

Anne D. Donaldson

Crossref

TFIIIC localizes budding yeast<i>ETC</i>sites to the nuclear periphery

http://dx.doi.org/10.1091/mbc.E11-04-0365

TFIIIC Localizes Budding Yeast ETC Sites to the Nuclear Periphery

Abstract

Similar works

Full text

Available Versions

LSU Scholarly Repository (Louisiana State Univ.)

Aberdeen University Research

Crossref