Gow, Neil A R

Lee, Keunsook

Munro, Carol A

Pietro, Rosemeire C L R

Preechasuth, Kanya

Santos, Emerson

Severi, Juliana A

Souza-Moreira, Tatiana M

Vilegas, Wagner

Molecules

English

In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors

Souza-Moreira, Tatiana M.

Severi, Juliana A.

Gow, Neil A.R.

Munro, Carol A.

Pietro, Rosemeire C.L.R.

Acervo Digital da Universidade Estadual Paulista

Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study

In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors.Department of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São PauloDepartment of Microbiology Institute of Medical Sciences University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZDDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São PauloDepartment of Pharmacy and Nutrition Centro de Ciências Agrárias Alto Universitário, Guararema, 29500-000, Alegre, ESDepartment of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São PauloDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São Paul

Souza-Moreira, Tatiana M. [UNESP]

Severi, Juliana A. [UNESP]

Santos, Emerson [UNESP]

Vilegas, Wagner [UNESP]

Pietro, Rosemeire C.L.R. [UNESP]

Repositório Institucional UNESP

In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level

Tatiana Souza-Moreira

Juliana Severi

Keunsook Lee

Kanya Preechasuth

Emerson Santos

Neil Gow

Carol Munro

Wagner Vilegas

Rosemeire Pietro

Multidisciplinary Digital Publishing Institute

Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study

The Open University

LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas

Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study

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Multidisciplinary Digital Publishing Institute

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LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas

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