This work was funded by the Neonatal Unit Endowment Fund, Aberdeen Maternity Hospital. RH is funded by a career researcher fellowship from NHS Research Scotland. SG was funded by the MRC Flagship PhD programme. We are grateful for the support of Dr Phil Cash and Aberdeen Proteomics, at University of Aberdeen, in completing this project. Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-018-24811-3.Peer reviewedPublisher PD

Berry, Susan

Collie-Duguid, Elaina S. R.

De Paiva Alves, Eduardo

El-Omar, Emad

Gibson, Sophie

Goddard, Mark

Hansen, Richard

Hold, Georgina L.

Karcher, Anne Marie

MacLaren, Andrew

Munro, Mike

Scientific Reports

English

Research Archive

Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin

Hyperbilirubinemia is so common in newborns as to be termed physiological. The most common bacteria involved in early-onset neonatal sepsis are Streptococcus agalactiae, commonly called Group B Streptococcus (GBS). Whilst previous studies show bilirubin has antioxidant properties and is beneficial in endotoxic shock, little thought has been given to whether bilirubin might have antibacterial properties. In this study, we performed a transcriptomic and proteomic assessment of GBS cultured in the presence/absence of bilirubin. Our analysis revealed that increasing levels of bilirubin (&gt;100 μmol/L) negatively correlated with GBS growth (18% reduction from 0-400 μmol/L on plate model, p &lt; 0.001; 33% reduction from 0-100 μmol/L in liquid model, p = 0.02). Transcriptome analysis demonstrated 19 differentially expressed genes, almost exclusively up-regulated in the presence of bilirubin. Proteomic analysis identified 12 differentially expressed proteins, half over-expressed in the presence of bilirubin. Functional analysis using Gene Ontology and KEGG pathways18 revealed a differential expression of genes involved in transport and carbohydrate metabolism, suggesting bilirubin may impact on substrate utilisation. The data improve our understanding of the mechanisms modulating GBS survival in neonatal hyperbilirubinemia and suggest physiological jaundice may have an evolutionary role in protection against early-onset neonatal sepsis.</p

Collie-Duguid, Elaina S.R.

University of Dundee Online Publications

Adaptive response of neonatal sepsis-derived Group B <i>Streptococcus </i>to bilirubin

https://discovery.dundee.ac.uk/files/101804638/s41598_018_24811_3.pdf

Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin

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University of Dundee Online Publications