ACKNOWLEDGEMENTS This work was funded by Aberdeen University Development Trust and Friends of Anchor. The University of Aberdeen is applying for a patent based on this work. Aberdeen University Development Trust (GrantNumber(s): DB10452-11) Friends of Anchor (GrantNumber(s): RS 2018 001)Peer reviewedPublisher PD

Antonopoulos, Aristotelis

Brewin, John N.

Cao, Huan

Davie, Adam

Dell, Anne

Girard, Louis-Pierre

Haslam, Stuart M

Henderson, Sadie

Mathur, Abhinav

Rees, David C.

Robertson, Charlotte

Vickers, Mark

Wong, Jin Hien

Wright, Sonja

British Journal of Haematology

English

PubMed

Red blood cells (RBCs) lose plasma membrane in the spleen as they age, but the cells and molecules involved are yet to be identified. Sickle cell disease and infection by Plasmodium falciparum cause oxidative stress that induces aggregates of cross-linked proteins with N-linked high-mannose glycans (HMGs). These glycans can be recognised by mannose-binding lectins, including the mannose receptor (CD206), expressed on macrophages and specialised phagocytic endothelial cells in the spleen to mediate the extravascular haemolysis characteristic of these diseases. We postulated this system might also mediate removal of molecules and membrane in healthy individuals. Surface expression of HMGs on RBCs from patients who had previously undergone splenectomy was therefore assessed: high levels were indeed observable as large membrane aggregates. Glycomic analysis by mass spectrometry identified a mixture of Man5-9GlcNAc2 structures. HMG levels correlated well with manual pit counts (r = 0.75–0.85). To assess further whether HMGs might act as a splenic reticuloendothelial function test, we measured levels on RBCs from patients with potential functional hyposplenism, some of whom exhibited high levels that may indicate risk of complications

Cao, H

Mathur, A

Robertson, C

Antonopoulos, A

Henderson, S

Girard, L-P

Wong, JH

Davie, A

Wright, S

Brewin, J

Rees, DC

Dell, A

Haslam, SM

Vickers, MA

Spiral - Imperial College Digital Repository

Measurement of erythrocyte membrane mannoses to assess splenic function

Red blood cells (RBCs) lose plasma membrane in the spleen as they age, but the cells and molecules involved are yet to be identified. Sickle cell disease and infection by Plasmodium falciparum cause oxidative stress that induces aggregates of cross‐linked proteins with N‐linked high‐mannose glycans (HMGs). These glycans can be recognised by mannose‐binding lectins, including the mannose receptor (CD206), expressed on macrophages and specialised phagocytic endothelial cells in the spleen to mediate the extravascular haemolysis characteristic of these diseases. We postulated this system might also mediate removal of molecules and membrane in healthy individuals. Surface expression of HMGs on RBCs from patients who had previously undergone splenectomy was therefore assessed: high levels were indeed observable as large membrane aggregates. Glycomic analysis by mass spectrometry identified a mixture of Man(5‐9)GlcNAc(2) structures. HMG levels correlated well with manual pit counts (r = 0.75–0.85). To assess further whether HMGs might act as a splenic reticuloendothelial function test, we measured levels on RBCs from patients with potential functional hyposplenism, some of whom exhibited high levels that may indicate risk of complications

Girard, Louis‐Pierre

Brewin, John

Haslam, Stuart M.

Vickers, Mark A.

PubMed Central

ACKNOWLEDGEMENTS This work was funded by Aberdeen University Development Trust and Friends of Anchor. The University of Aberdeen is applying for a patent based on this work. Aberdeen University Development Trust (GrantNumber(s): DB10452-11) Friends of Anchor (GrantNumber(s): RS 2018 001)Peer reviewe

Aberdeen University Research

http://spiral.imperial.ac.uk/bitstream/10044/1/96613/2/Br%20J%20Haematol%20-%202022%20-%20Cao%20-%20Measurement%20of%20erythrocyte%20membrane%20mannoses%20to%20assess%20splenic%20function.pdf

Measurement of erythrocyte membrane mannoses to assess splenic function

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