The observation was carried out in twenty fishponds in the surrounding of Vodňany and seven fisponds in the surrounding of Třeboň. The aim of the work was to evaluete the quality and quantity of the zooplankton depending on fish production. The samples were obtained and the observations were realized during the 4 years in Vodňany fishponds area and during the 2 years in Třeboň fishponds area. At first the samples were collected and data about the observed localities were organized. These data should explain changes in the aquatic ecosystems of these fishponds areas during the last decades