Genetic transformation of embryogenic cultures of Norway spruce (\kur{Picea abies})

Abstract

The aim of this study was to develop a transformation procedure for embryonic cultures of Norway spruce (Picea abies) using particle bombardment. Selection of the transgenic tissue was based on the antibiotic resistance conferring by the nptII gene, in combination with the antibiotic kanamycin. Expression of the gus gene was confirmed by histochemical analysis