DNA značená aminofenylem a nitrofenylem. Syntéza pomocí enzymatické inkorporace modifikovaných nukleosid trifosfátů, studium elektrochemických vlastností takto značené DNA

Abstract

We employed single step aqueous phase Suzuki-Miyaura cross-coupling reactions of halogenated 2'-deoxynucleoside 5'-triphosphates with 3-aminophenyl and 3-nitrophenylboronic acid for synthesis of modified nucleoside triphosphates (dNTPs). These dNTPs were then enzymatically incorporated into DNA in Primer extension experiment (PEX). Electrochemical detection using square-wave voltammetry of single-strand modified oligonucleotides (ONs) bearing 3-aminophenyl and 3-nitrophenyl tag demonstated excellent utilization in labeling of DNA