Influence of Isochinolinic A-Fraction (Phenolic CHCl3 Insoluble Cl- > Salts) Alkaloids from Corydalis Cava(Fumariaceae) on Cholinesterases

Abstract

From 12 kg of Corydalis cava bulbs, ethanolic extract was prepared. From this extract, pure alkaloid concentrates. Tertiary bases of average basicity (extract A, 200g) were disolved in hydrochloric acid and two fractions were separated, based on solubility in chloroform. Both fractions were further resoluted to phenolic and non-phenolic bases. Their structure was determined by MS and NMR. Effectivity of obtained compounds was evaluated in vitro, or determined by standard IC50 inhibition test. Human erythrocytal AChE and serum BuChE were used as source ov AChE. Activity was measured spectrophotometrically using standard Elman method. Results were compared to values of IC50 of reference compounds – terapeutic inhibitors (donepezil, rivastigmin, galanthamin, huperzin A)