We describe an adaptive optics technique for two-photon microscopy in which
the deformable mirror used for aberration compensation is positioned in a plane
conjugate to the plane of the aberration. We demonstrate in a
proof-of-principle experiment that this technique yields a large field of view
advantage in comparison to standard pupil-conjugate adaptive optics. Further,
we show that the extended field of view in conjugate AO is maintained over a
relatively large axial translation of the deformable mirror with respect to the
conjugate plane. We conclude with a discussion of limitations and prospects for
the conjugate AO technique in two-photon biological microscopy
