Cells modify their volume in response to changes in osmotic pressure but it
is usually assumed that other active shape variations do not involve
significant volume fluctuations. Here we report experiments demonstrating that
water transport in and out of the cell is needed for the formation of blebs,
commonly observed protrusions in the plasma membrane driven by cortex
contraction. We develop and simulate a model of fluid mediated membrane-cortex
deformations and show that a permeable membrane is necessary for bleb formation
which is otherwise impaired. Taken together our experimental and theoretical
results emphasize the subtle balance between hydrodynamics and elasticity in
actively driven cell morphological changes.Comment: Phys. Rev. Lett. in press. 13 pages 4 figures, 9 supplementary
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