We describe how a single-particle tracking experiment should be designed in
order for its recorded trajectories to contain the most information about a
tracked particle's diffusion coefficient. The precision of estimators for the
diffusion coefficient is affected by motion blur, limited photon statistics,
and the length of recorded time-series. We demonstrate for a particle
undergoing free diffusion that precision is negligibly affected by motion blur
in typical experiments, while optimizing photon counts and the number of
recorded frames is the key to precision. Building on these results, we describe
for a wide range of experimental scenarios how to choose experimental
parameters in order to optimize the precision. Generally, one should choose
quantity over quality: experiments should be designed to maximize the number of
frames recorded in a time-series, even if this means lower information content
in individual frames
