Serologisch onderzoek bij Fusarium oxysporum

Abstract

Rabbits were immunized with different preparations of F. oxysporum.Antibodies against culture fluid, homogenized mycelium, mycelial extracts and microconidial suspensions reacted in precipitin tests with both culture fluids and mycelium extracts.Cross reactions between formae speciales were very common. The gel diffusion precipitin test proved most suitable to differentiate formae speciales . Most sera did not specifically differentiate formae speciales . However some reacted highly specifically.Concerning the character of the antigens it was concluded that the mycelium contained glycoproteins. The glycoproteins split up into polysaccharides and various proteins both during autolysis in the cultures and during extraction of the mycelium.Rabbits only produced antibodies against proteins after injection with culture liquids or with saline extracts of mycelium. These proteins varied widely in physical properties and it was very difficult to reproduce the same proteins, even by the same extraction procedure. The polysaccharides were much more stablebut antibodies against them could be obtained only by immunization with microconidia. Probably these polysaccharides were present on the surface of the cell wall of the microconidia.The study demonstrated that the polysaccharides from the glycoproteins of Fusarium oxysporum were haptens