Polyherbal Extract (Soy, Brown Rice and Coconut Water) Improves Endothelial Damage in Diabetes Mellitus Through Modulation of Oxidative Stress and Endothelial Progenitor Cells

Abstract

This study aims to investigate the effects of poliherbal extract (soybeans, brown rice and coconut water) in repairing endothelial damage in diabetes mellitus through oxidative stress modulation and endothelial progenitor cells. A total of thirty Wistar rats will be divided into five study groups (n= 6 each), including the control group (without any treatment), the diabetic mouse rats group, the diabetic mouse rats administered various dosage poliherbal extracts (0.067 grams/200 grams body weight 0.135 gram/200 gram of body weight, or 0.270 gram/200 gram body weight). Giving poliherbal was done for 28 days. MDA and SOD levels in serum were analyzed by spectrophotometric technique.The amount of circulating endothelial cells and endothelial progenitor cells were analyzed by flow cytometry. The MDA levels was increased in the diabetes mellitus group compared to the control group. Poliherbal lowered levels of MDA than diabetes mellitus group. There was a decrease in SOD levels in the diabetes mellitus group compared to controls. Provision of poliherbal extract increased levels of SOD compared to diabetes mellitus group. The number of circulating endothelial cells increased in the diabetes mellitus group compared with the control group, this increase can be suppressed by all groups given the poliherbal extract. There was an increase in the number of endothelial progenitor cells in the diabetes mellitus group compared with the control group. The higher the dose of the extract given the lower the number of endothelial progenitor cells. It was concluded that the administration of poliherbal extract could inhibit oxidative stress and endothelial damage in diabetic mellitus rats through modulation of SOD antioxidants as well as modulation of endothelial progenitor cells