The modern ZP maize hybrids breeding at the Maize Research Institute, Zemun Polje, begun during the fifties of the 20(th) century. Collecting of the initial material was the first step in conducting maize breeding programs. The maize inbred line-developing programs from three local populations has initiated in 1953. That was beginning of breeding first cycles ZP maize hybrids. From that period up to present time, we have had five generations of ZP maize hybrids. Each generation has characterized by introduction of the new potentially higher yielding hybrids and with better other agronomics characteristics. According to results from different studies, the genetic yield potential of ZP maize hybrids over the last 40 years amounted to 100 kg.ha(-1) per year. ZP 755 was the first registered maize hybrid in the country in 1964. Total 499 ZP maize hybrids have released by the State Government Commission's since 1964 and 99 ZP maize hybrids have registered in 15 countries. Since the seventies biotechnology methods have been applied in the breeding programs. The main part of biotechnology work is based on the molecular marker application for determining genetic diversity, QTL mapping for drought tolerance and identification of chromosome regions harbouring QTLs for economically important traits (yield, kernel oil content)