Experiments were conducted with three biofloc treatments and one control in triplicate in 1000 ltr capacity indoor tanks and water level filled up to 600 ltr. Enhanced shrimp growth was noticed in biofloc treatment tanks and a significant difference in the final average body weight of (15.92±0.07g) was found in the wheat flour treated shrimps than those of control group shrimp. The Feed Conversion Ratio differs significantly (P<0.05) between biofloc treatment group and control groups. Lowest FCR (0.5±0.07) was recorded in wheat flour as carbohydrate source biofloc treatment during the experiment. Highest Specific Growth Rate (4.59) was observed in the wheat flour treatment groups than that of other treatments and control groups. Wheat flour utilization as carbohydrate source to biofloc development for rearing of L. vannamei was proved to be the best option among all treatments. All the carbohydrate sources (wheat flour, tapioca flour and molasses) utilized for biofloc treatments indicated highest growth than control treatment
