The sugar in the blood of crustacea

 represents an energy source for tissue

metabolism as is the rule in other animals.

The wide range  of composition of blood

values obtained for reducing substances

in crustacean blood suggest that the physiological

condition of the animal influences-the-

'blood sugar values'

Nammalwar, P

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more suspICiOn, superstition" and: ~viih 'old RreS]lmption , IlL Indi.a _ ,yealllllg , of Iambs has' , not, beell;-takeil up serior~l:( fJ.s is being poile with various flocks abroad .. , ./ !he . revie~v points .out the." dearth of ..uuormatlon on our natIve breerls and theIr j &osses with exotic breeds and ' about tbe insufficient attempts ' for studying the use-fulneSs of "'eaning in our country. M . NARAYANASWAMY KDDC, Central _Office, Bangalore 560027.-Received , : 23 Sep.tember, 1977, 1 R. M. Acharya and S. J. S. Bawa, Indian, J. Agri. Sci. 41, 572, 1971. 2: C .. R:. Ba.rn!co~t: P. F. Murray. E. M . Roberts and ~G. S. WiISOll, J . Agri. Sci., 4],577,1957. 8 M. J. Burrics and llallglls, J. Agri. Sci. 14, 1.86; 1955. c. ' 41 J. E., Coop, J. ' Agri. Sci., 40, 311, 1950. i -J. 'M. Doney and J. Munro, Anim. Prod. 4, 215, 1962. 6 J. B. Owen', J: Agri. Sci. 40, 387, 1957. 7 b. ,D. Sucker and J. C. M. Trail, J. Agric. Sci., 6,7, 93, I ?66 .. , O. N:Seth;M.D, Pandey and A. Roy, 4S, 9, 1971. . Indiall J. Anim S ci. _ 9 V. K. Singh, S. B. : Tiwari, L. B. Singh and 1., Honmode, Indian, Vel, 1. SO, 1199, 1973. ]0 K . Sugai and ,K ... Teramato. s ci. Rep, no, It, Fac'. AgrfG. lKoy~ma, Univ, (Vi'de. Anim. Breed, A,bSi;1'. 26 , : 409). 1958. 11 W: Thonlso'n and ~A: M. Thomson, Brit, 1. NUlr. , 7, 263, 1951'. 12 J. D. Wardrop and J, B. Coombe, J . Agric. Sc;. 54, 140, 1960. . 13 W. L, Willia.ms. The diseases of gen ital organs of domes t i c animals. 3r.d Ed. Ethel William , Plimto"n. *orcestei , Mass: 1947. .. . Blood Sugar Estimation in the Estuarine Crab Scylla serrata (Forskal)* The sugar in, tire blood 'of crustacea ' represents an eUQl'gy Sd lll'Ce fOI: tissue metabolism as is lhe rule in other animals. Tile wide range ' of COl)lpositio,n of blood ~ .' • j •. . ", valtles 'obtained 'for reduCing substances :--:-: __ ~.__ 1 .. !. , '* This stupy formed a ,s ~a part of th~ dissertation ' su~mitted in t,he ,partialrulfilrnent oC the rcquirem~nts Cdr' tl}e M,Sc. ! degree from Annamalai Uni've-rsity, ' 1967, ' , v6I:., 44, NO. 12 'j' • ~ - a_ ~ . in cl'llstacean blood suggest that the physio-logical condition of the animal iIlOuences-the-'blooel sugar values'. ' ~ome aspects of biologY., fishery" histo-chemistry, physiology, rate of survival aneI culture in the estuarine crab Scylla serrata (Forskal) were available from earlier litera-ture'-". As there was no emphaSis on blo ad sugar estimatinn in this species in earlier accounts, ,an attempt' has heen made in the present communication to give: some information, on, this subject, , The estuarine crabs of the species Scylla serrata (Forskal) 'were collected alive from the Vellar Estuary of Porto Novo (C11° 29°N 79° 49°E) on the east coast of In(lia. In 'an experimeut, 1 ml of blood from the living crab S. serrata was mixed witll ' 7 mI of water in a fl ask. 1 mL of 10 % soluLion of zinc sulphate was adj:led to the aliquot. While shaking coutinuously, 1 ml of 0.5 N NaOH was added. The flask was closed with a stopper: shaked well and filtered, for a few minlltes through a clry fllter paper . Protein was precipitated with barium llydroxideanrl the protein free filLrate was collected. 5 m!. of copper reagent was poured into a large test tube and 5011 of protein free filLrate was then added to it. After prope,' shaicing, the Lube was covered with a small funnel and kept in a boiling water bath for 15 minutes. Then it was cooled under the tap water. When the temperature of the solu-tion came down to about 40°C, 1 ml of: 5N H,SO. was added to dissolve all t11e copper oxide produced. After abotit ,2 minutes, it was titrated with 0.005 % sodiun'\, thiosulphate solution, using ' starch as indi-cator. Towards '.the end of the titration a blank with 5 m!. of reagents was used and no blood flItrate was used. From the diffe-rence between the titre .values of the blank and experimental sample, the glucose equi-valent was determinea, From this, the percentage of gIucos~ contents in the hlo9(\l was calculated. TABLE 1: Blood sugar eontent in Scylla serrata (ForskaI) -------------------------------~------.---------M1. of 0.005 Vol. Difference Na2SaOa Glucose • Na2S20S used equivalant equivalent ----------------------------------------------~-Blank 124 mg/lOO ml 4.5 4.5 (whole blood) I· Experiment 16.6 ------------------------~-------------- -----.---It was estimated from the present experi-ment that sugar content of the blood in S. serrala was i'2,4 mg/ilili ml. Scylla sen'ata (Forskal) which can tole-rate a wide range of salinity has been found to regulate its blood calcium and sugar with the dilution of the medium. The fan of blood sugar level in fasting Carcinlls maenas outside the moulting period as observed by Kleinholz et al .. , substantiated the present findings in S. serratan . I am grateful to Prof. R. V. Seshiya, (Late) Director and Dr. V. K. Venugopalan, Reader, Department of Marine Biology (Annamalai University), Porto-Novo for their keen inte-rest and encouragement. .P. NAMMALWAR I) p~ G. Renganekar, Zool. J. Linn. Soc. 57 (1), 75.84., 1975. ~ P. G. Reng,anekar, et. at. Histochemicalobserva. t'tons on the occuuence 0\ g\yco\'jt,\c am\. pentose potycyc1e enzyme in hepatapancreas and their possibilty to eyes talk factors. L. Mikrosk Anat. Forsch (I.eipz), 89 (1) : 128. 142, 1975. 7 K. R. Menon and Sivadas, Blood sugar regulation in the crab Scylla serrata 1. Effect of injection of eye.stalk extract. Indian J. Exptl. Bioi. 5, 176.178, 1967. 8 K. R. Menon and Sivadas, Hormonal control of blood calcium and sugar levels in the estuarine crab Scylla serrato (Forskal). Proc. Indian Academy Sciences. Vol. LXVII, Section B. NO.3: 132.138. 1968. 9 K. R. Menon, Mechanism of salinity tolerance in an estuarine crab Scylla serrata (Forskal), 1968. (In Press) 10 R. B. Vasudev and H. G. Kewalramani, Trans. port of the common crab (Scylla serrata) in living condition. Indian. J. Fish. 7, 169.173. 1960. ICAR Regional Centre for Central Marine It M. Kathirvel, Culture of other crustaceans. Fisheries Research Institute, Marine Fisheries P. O. Mandapam Camp, Tamilnadu. Received: 10 September, 1977 Revised: 17 August, 1978 1 Talbot H. Waterman, The physiology of crustacea Vol. I. Metabolism and . growth, 1960, p. 141~159. Academic Press, New York. 2 D. D. Rekha, SOine aspects of biology of the marine crab, Scylla serrata ,(Forskal). Ph.D. thesis, University of Bombay, 1968. a D. Rajasingh Joel, Studies on the bio1ogy and fisheries of the edible Portunid crabs of the Pu1icat Lake. Ph.D. Thesis, University of Madras. 1973. .. P. G. Renganekar, J. Anim. M orph. Physiol., I, 62.64, 1954 .. 544 Summer Inst itute. in Coastal aquaculture. Central Marine Fisheries Research Institute, Cochin.18. 1974. 11 L. M. Kleinholz, V. 1. Havel and R. Reicbart, Studies in the regulation of blood sugar concen. tration in crustaceans. BioI. Bull, 99, : 454.468, 1950. Eftects of Growth Regulators on Growth Development and Yield of Abelmoschus eswlentns (L) Moench (Lady's finger). The use of growth regnlators for con-trolling frui~ set and maturation, altering fruit size and improving its quality and development has become an important tool in the hands of agriculturists. Vadous SCIENCE AND CULTURE, DECEMBER, 1978 

Blood Sugar Estimation in the Estuarine Crab Scylla serrata (Forskal)

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Blood Sugar Estimation in the Estuarine Crab Scylla serrata (Forskal)

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