Advanced Biotechnological research is largely depended on the genome analysis and
recombinant DNA technology. Good quality nucleic acid is an essential prerequisite for
consistent results in most of the down stream applications in the genome analysis and
recombinant DNA technology. The general principle underlying the isolation of nucleic acids
is common with few modifications depending on the type of nucleic acids being isolated.
The type of the nucleic acid intending to isolate is to be made free from the other biological
macromolecules and cell debris. This is achieved by properly lysing the cell wall or cell
membrane as the case may be and by selectively denaturing the other macromolecules like
proteins