Electrophoresis of eye lens protei ns of oil sardine and

mackerel showed separation of  proteins into three and four

components, indicating the heterogenous nature of the population

Dhulkhed, M H

Rao, S Narasimha

CMFRI Digital Repository

ELECTROPHORETIC CHARACTERISTICS OF OIL SARDINE (SARDINELLA LONGICEPS) AND MACKEREL (RASTRELLIGER KANAGURTA) EYE LENS PROTEINS S: NARASIMHA RA O Department o[ Biochem;Hry, I<.asturba Medical College, Manga/ore. AND M. H. DHULKHED Centra l A:orine Fisileries Re~ earch Institute: Sub-'iration, Manga/ore. Electrophoresis of eye lens protei ns of oil sardine and mackerel showed separation 01 proteins into three anJ four components, indicating the heterogenoou> nature of the pcpulation. J ~TRODU(,TlON The sero logical and bi ochemica l app-roadl fo r identifying fish species and p opulatiOn. has been excellently reviewed by Ligny (1969). The search for intra-speci fic differences by electrophoresis of the eye lens proteins was initiated by Smith ( 1962 and 1965). The oil sardine and mackerel constiture commercially impo-rtant fisheries of India. Earlier studies were mainly related to their morphometric and meristic cOllnts (Balakrishnan , 1965; Prabhu and Dhulkhed, 1972). To identify the different gro ups, if any, preliminary in-vestigations on the electrophoretic chara-cleri;tics of the eye len s proteins of these fish", were conducted at. Mangalore during Decem ber, 1973 to Februa,y, 1974. MATERIALS AND METHODS Eye lens of both the sides of oi l VOL · 11 No. 1976 • sardine and mackerel (10 nos . each) cQllected in fresh co ndition between Malpe in the north and Ca nnanore in the south, were first removed. The nuclei were then squeezed out, cleared of aqueous humor and dried on a blotting paper. The nuclei were stored in a refr igerator for not more than 48 ho ur5. The two nuclei of each sardine and mackerel were placed separately in test tubes containing 0.5 m!. and I m!. of 0 .9% sodium chloride res-pectively and were thoroughly minced by means of a glass rod till the extracts became evenly m ilky . The extracts were kept in a refrigerato r and cer.trifuged the next day. An application of 31-'1. of eye lens extract was mad~ on a cellogel strip and the method described earlier for electro phoresis (Dhulkhed and R ao, 1976) was followed. The strips were stained by Amido black and read on the densitometer. 13 ELECTROPHORETIC CHARACTERISTICS OF OIL SARDINE (SARDINELLA LONGICEPS) AND MACKEREL (RASTRELLIGER KANAGURTA) EYE LENS PROTEINS S: NARASIMHA RA O Department o[ Biochem;Hry, I<.asturba Medical College, Manga/ore. AND M. H. DHULKHED Centra l A:orine Fisileries Re~ earch Institute: Sub-'iration, Manga/ore. Electrophoresis of eye lens protei ns of oil sardine and mackerel showed separation 01 proteins into three anJ four components, indicating the heterogenoou> nature of the pcpulation. J ~TRODU(,TlON The sero logical and bi ochemica l app-roadl fo r identifying fish species and p opulatiOn. has been excellently reviewed by Ligny (1969). The search for intra-speci fic differences by electrophoresis of the eye lens proteins was initiated by Smith ( 1962 and 1965). The oil sardine and mackerel constiture commercially impo-rtant fisheries of India. Earlier studies were mainly related to their morphometric and meristic cOllnts (Balakrishnan , 1965; Prabhu and Dhulkhed, 1972). To identify the different gro ups, if any, preliminary in-vestigations on the electrophoretic chara-cleri;tics of the eye len s proteins of these fish", were conducted at. Mangalore during Decem ber, 1973 to Februa,y, 1974. MATERIALS AND METHODS Eye lens of both the sides of oi l VOL · 11 No. 1976 • sardine and mackerel (10 nos . each) cQllected in fresh co ndition between Malpe in the north and Ca nnanore in the south, were first removed. The nuclei were then squeezed out, cleared of aqueous humor and dried on a blotting paper. The nuclei were stored in a refr igerator for not more than 48 ho ur5. The two nuclei of each sardine and mackerel were placed separately in test tubes containing 0.5 m!. and I m!. of 0 .9% sodium chloride res-pectively and were thoroughly minced by means of a glass rod till the extracts became evenly m ilky . The extracts were kept in a refrigerato r and cer.trifuged the next day. An application of 31-'1. of eye lens extract was mad~ on a cellogel strip and the method described earlier for electro phoresis (Dhulkhed and R ao, 1976) was followed. The strips were stained by Amido black and read on the densitometer. 13 

Electrophoretic characteristics of oil sardine (Sardinella longiceps) and mackerel (Rastrelliger kanagurta) eye lens proteins

Electrophoresis of eye lens proteins of oil sardine and mackerel showed separation of proteins into three and four components, indicating the heterogeneous nature of the population

Narasimha Rao, S.

Dhulkhed, M.H.

Aquatic Commons

ElECTROPHORETIC CHARACTERISTICS Of OIL 
SARDINE (SARDINELl.A LONGICEPS) AND MACKEREL 
(RASTRELLIGER KANAGURTA) EYE lENS PROTEINS 
S. N ARASIMHA RAO 
Department of Biochemistry, Kasturba Medical College, Mango/ore. 
AND 
M. H. DHULKHED 
Central 1\:arine Fisheries Research Institute: Sub-station, Mango/ore. 
Electrophoresis of eye lens proteins of oil sardine and 
mackerel showed separation of proteins into three and four 
components, indicating the heterogen..:ous nature of the pc pulation. 
INTRODUCTION 
The serological and biochemical app-
roach for identifying fish species and 
populations has been excellently reviewed 
by Ligny (1969). The search for intra-
specific differences by electrophoresis of 
the eye lens proteins was initiated by 
Smith (1962 and 1965). The oil sardine 
and mackerel constitute commercially impo-
rtant fisheries of India. Earlier studies were 
mainly reiated to their m::>rphometric and 
meristic counts (Balakrishnan, 1965; Prabhu 
and Dhulkhed, 1972). To identify the 
different groups, if any, preliminary in-
vestigations on the electrophoretic chara-
cteristics of the eye lens proteins of these 
fishes were conducted at Mangalore during 
December, 1973 to Februa£y, 1974. 
MATERIALS AND METHODS 
Eye lens of both the sides of oil 
VoL 13 No. 1 1976 
sardine and mackerel (10 nos. each) 
collected in fresh condition between Malpe 
in the north and Cannanore in the south, 
were first removed. The nuclei were then 
squeezed out, cleared of aqueous humor 
and dried on a blotting paper. The 
nuclei were stored in a refrigerator for 
not more than 48 hours. The two nuclei 
of each sardine and mackerel were placed 
separately in test tubes containing 0.5 ml. 
and 1 ml. of 0.9% sodium chloride res-
pectively and were thoroughly minced by 
means of a glass roc! till the extracts 
became evenly milky .. The extracts were 
kept in a refrigerator and cer,trifuged 
the next day. An application of 3 ft l. 
of eye lens extract was made on a 
cellogel strip and the method described 
earlier for electrophoresis (Dhulkhed and 
Rao, 1976) was followed. The strips were 
stained by Amido black and read on the 
densitometer. 
13 
i 
II 
·! 
Fig. 1. Graphic representation of the electrophoretic separation of eye lens proteins 
of oil sardine. A. three components; B. four cmnponents. 
REsULTS AND DISCUSSION 
The electrophoresis indicated separa-
tion of soluble eye lens proteins into 
three or four components in the case of 
both oil sardine and mackerel. Though 
the electrophoretograms (Figs. 1-2) of these 
fishes revealed distinct number of com-
ponents, it could still be seen that the 
resolution of the eye lens proteins of 
oil sardine was . more clear than those of 
mackereL The pattern of protein con-
centration curves of the oil sardine and 
mackerel with three components is almost 
identical, whereas slight variations were 
14 
descernible in the four component group. 
However, in the case of mackerel, the 
protein concentration curve was rather 
diffused. 
Although the pattern variations in the 
components within the species appear to 
be minor, it could still be inferred that 
the population of oil sardine and mackerel 
is heterogeneous. The presence of an extra 
component among these fishes could 
reasonably be attributed to genetic diffe-
rences as observed by Smith (1962) m 
the case of bluefin tuna and kelp bass. 
Fish. Techno!. 
Narasimfta 'Rao & 'Dfiulfilied: Electrop~aretic cfiaracteristics of eye lens prote~ns; 
Fig. 2. Graphic representation of the electrophoretic separation of eye lens proteins 
of mackerel. A. three components; B. four components. 
However, it is considered- that more 
detailed investigations of samples covering 
wider areas would be necessary to establish 
the intraspecific differences. 
AcKNOWLEDGEMENT 
The authors thank the Principal, 
Kasturba Medical College, Mangalore for 
extending all the facilities and Drs. K. V. 
Sekharan and S. Ramamurthy for their 
useful suggestions. 
REFERENCES 
Balakrishnan, V. 1965. Indian J. Fish., 
12, 1 : 60. 
VoL 13 No. 1 1976 
Dhulkhed, M. H. anj S. Rao. 1976 
Fish. Techno!., 13, 1 
Ligny W. de. 1969. Oceanogr. Mar. Bioi. 
Ann. Rev., H. Barnes, George Allen 
and Unwin Ltd., London, 1 : 411. 
Prabhu, M.S. and M. H. Dhulkhed. 1972. 
Mahasagar; 5, 2 : 27. 
Smith, A. C. 1 962. Calif. Fish and Game; 
48, 3 : 199. 
Smith, A. C. 1965. Calif. Fish and Game: 
51, 3 : 13l. 
15 


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Electrophoretic characteristics of oil sardine (Sardinella longiceps) and mackerel (Rastrelliger kanagurta) eye lens proteins

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