This study was aimed for the detection of Vibrio
parahaemolyticus by biochemical and molecular methods in
seafood samples collected from the markets of Cochin
located at the southwest coast of India.Atotal of seventy-two
V. parahaemolyticus cultures were isolated by selecting
sucrose and cellobiose non-fermenting colonies. All the
biochemically confirmed strains were found to have 368-bp
toxR gene fragment, while an additional 24% of the samples
were confirmed as V. parahaemolyticus by toxR based
polymerase chain reaction (PCR) from enrichment broths