The instruments used for the DSL studies were scientific echo-sounder for
detection, Isacc's Kid Midwater trawl for sampling mesopelagics and the trawl sonde
to lower the IKMT to the exact depth at which the DSL appeared. Opening of the
IKMT and the temperature of the seawater at which the DSL appeared was also
recorded on the trawl sonde system. The echoscope connected to the echosounder
showed the DSL in different colours depending upon its density. The continuous
monitoring on echogram revealed that the DSL observed at surface during night and
at 600 m depth during day