Isolation and culture of protoplasts from agar yielding seaweed Gracilaria edulis (Gmelin) Silva

Abstract

Viable protoplasts werl! isokn~d [rom cuJt ure~ thallus of ag<J.r y~ilding red ::)~aweed GI"Gciiarill eduhs using an cnzymc mixture of 3% ONOZUKt"\ R-IO cdlulase 3% R-\ 0 maceroz),lll c, 1% agarasc and 1% pectinase in a seawr!cd protoplast washing mediulI1 (SPW) containing seawater and 1 M mannitol. After repeated washing wiih sterile ml:dium, the pro~op l il $IS were maintained in light and attempts were made on eJ..::ctrofllsioll of protopl;J$ts