The vitamins, though required in small amounts in the
diet, play major roles in growth, physiology and metabolism of
the animal. Their absence in the diet causes major deficiency
syndromes. Also, the vitamins should be present in optimum
levels in the diet; any excess in certain vitamins in the diet
causes pathological symptoms and inhibit growth. Therefore,
the determination of the amount of different vitamins present
in the diet is very important
