Tor malabaricus (Jerdon) is a mahseer species endemic to the Western Ghats. Since its original description, taxonomic position of the species has been extremely confusing. In the present study, Random Amplified polymorphic DNA (RAPD) markers were used to determine the taxonomic status of T.malabaricus collected from Balamore River, Tamil Nadu, India, by comparing its RAPD profile with that of Tor khudreee.15 random oligodecamers were used to amplify DNA from Tor malabaricus and Tor khudree (n=30 each) collected from two geographically isolated localities and a total of 119 amphicons were detected