De Wet, Heidi

English

Cape Town University OpenUCT

2- and 8-azido photoaffinity probes. 1. Enzymatic synthesis, characterization, and biological properties of 2- and 8-azido photoprobes of 2-5A and photolabeling of 2-5A binding proteins.

2'-3'-O-(2,4,6-trinitrophenyl)-8 azido-AMP and -ATP photolabel Lys492 at the active site of sarcoplasmic reticulum ea2 +_ ATPase.

2',3'-O-(2,4,6-trinitrophenyl)-8-azido-adenosine mono-, di-, and triphosphates as photoaffinity probes of the Ca2+-ATPase of sarcoplasmic reticulum. Regulatorylsuperfluorescent nucleotides label the catalytiC site with high efficiency.

A bacterial antibiotic-resistance gene that complements the human multidrug resistance p-glycoprotein gene.

A rapid and sensitive method for the qLiantitation of microgram quantities of protein utilzing the principle of protein-dye binding.

A retrovirus carrying an MDR1 cDNA confers multidrug resistance and polarized expression of P-glycoprotein

A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

A view of sur/KIR6.X, K(ATP) channels.

A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein.

Affiiniity labeling of the ATP bindiing site of Ca 2+-transportiing ATPase of sarcoplasmic retiiculum by adenosine triphosphopyridoxal: identitiication of the reactive Iysyl residue.

Alteration of substrate specificity by mutations at the His61 position in predicted transmembrane domain 1 of human MDR1-P-glycoprotein.

Amplification or the multidrug resistance gene in some chloroquine-resistant isolates of P.fa/ciparum.

Analysis of the intron-exon organization of the human multidrug-resistance protein gene (MRP) and alternative spliCing of its mRNA

ATP hydrolysis cycles and mechanism in p glycoprotein and CFrR.

ATP hydrolysis cycles and the gating of CFfR cr channels.

ATP-binding properties of P-plycoprotein from multidrug-resistant KB cells.

ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p.

Binding protein-independent histidine prermease mutants.

Biochemistry of multldrug resistance mediated by the multidrug transporter.

Biological activities and spectroscopic properties of chromophoric and fluorescent analogs of adenine nucleoside and nucleotides, 2',3'-0-(2,4,6 trinitrocydohexadienylidene) adenosine derivatives.

Both P-glycoprotein nucleotide-binding sites are catalytically active.

Ca 2+ -dependent conformational change of the ATP-binding site of Ca2+-transporting ATPase of sarcoplasmic reticulum as revealed by an alteration of the target-site specificity of adenosine triphosphopyridoxal.

Catalytic mechanism of p-glycoprotein.

Chanes F. Kettering Prize. P-glycoprotein and resistance to anticancer drugs.

Characterization of phosphorylation-defective mutants of human p glycoprotein expressed in mammalian cells.

Characterization of the azidopine and vinblastine binding site of P-plycoprotein.

Clinical relevance of transmembrane drug efflux as a mechanism of multidrug resistance.

Competition of hydrophobic peptides, cytotoxic drugs, and chemosensitizers on a common p-glycoprotein pharmacophore as revealed by its ATPase acitivy.

Competitive and non-competitive inhibition of the rnultidrug-resistance-associated p-glycoprotein ATPases - futher experimental evidence for a multisite model.

Conformational changes of p glycoprotein by nucleotide binding.

Correlation between the affinity of flavonoids binding to the cytosolic site of Leishmania tropica multidrug transporter and their efficiency to revert parasite resistance to daunomycin.

Covalent inhibitors of P-glycoprotein ATPase activity.

Covalent modification of the nucleotide binding domains of cystic fibrosis transmembrane conductance regulator.

Crystal and molecular structure of 8-bromoguanosine and 8-bromoadensoine, two purine nucleosides in the syn confonnation.

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.

Dissection of drug-binding-induced conformational changes in p-glycoprotein.

Distantly related sequences in the a. and p-subunits of AlP-synthase, myosin, kinases and other AlP-requiring enzymes and a common nucleotide binding fold.

Domain mapping of the photoaffinity drug-binding sites in P-glycoprotein encoded by mouse mdr1b.

Drug binding and nucleotide hydrolyzability are essential requirements in the vanadate-induced inhibition of the human p-glycoprotein ATPase.

Drug-stimulated ATPase activity of human p glycoprotein requires movement between transmembrane segments 6 and 12.

Drug-stimulated nucleotide trapping in the human multidrug transporter MDR1. Cooperation of the nucleotide binding domains.

Effect of quercetin on Hoechst 33342 transport by purified and reconstituted

Effects of steroids and verapamil on P-glycoprotein ATPase activity: progesterone, desoxycorticosterone, corticosterone and verapamil are mutually non-exclusive modulators.

Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima.

Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein.

Expression of the gene for multidrug-resistance-associated protein and outcome in patients with neuroblastoma. The New England Joumal of Medicine.

Flavonoid-related modulators of multidrug resistance:synthesis, pharmacological activity, and structure-activity relationships.

Fragmentation of polypeptides by enzymatic methods. As in Practical protein chemistry - a handbook. Edited Darbre, A 122-148. Published by

Function, evolution and structure of multidrug resistance protein (MRP).

Functional and physical interactions between partial molecules of STE6, a yeast ATP-binding cassette protein.

Functional complementation of yeast ste6 by a mammalian multidrug resistance mdr gene.

Functional evidence that transmembrane 12 and the loop between transmembrane 11 and 12 form part of the drug binding domain in P-glycoprotein encoded by MDR1.

Functional expression of the multidrug resistance-associated protein in the yeast Saccharomycescerevisiae.

Functional multidrug resistance protein (MRP1) lacking the N-terminal transmembrane domain.

Functional reconstitution of p-glycoprotein reveals an apparent near stoichiometric drug transport to ATP hydrolysis.

Genomic organization of the human multidrug resistance. (MDR1) gene and origin of P glycoproteins.

Heavy metal resistance: A new role for P glycoproteins in Leishmania.

Hepatocyte-specifiC expression of the human MDR3 p-glycoprotein gene restores the biliary phosphatidylcholine excretion absent in MDR2 (-1-) mice.

Human p-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state.

Identification and characterization of SNQ2, a new multidrug ATP binding cassette transporter of the yeast plasma membrane.

Identification of amino acids modified by the bifunctional affinity label 5'-(p-(fluorosulfonyl)benzoyl)-8-azidoadenosine in the reduced coenzyme regulatory site of bovine liver glutamate dehydrogenase.

Identification of drug interaction sites in p-glycoprotein.

Identification of p-glycoprotein mutations causing a loss of steroid recognition and transport.

Identification of the cyclosporin-binding site in p-glycoprotein.

Identification of the cystiC fibrosis gene: cloning and characterization of complementary

Identification of the domains of photoincorportaion of the 3'-and 7-benzophenone analogues of taxol in the carboxyl-terminal half of murine mdr1 b p-glycoprotein.

Inhibition of P-glycoprotein ATPase activity by beryllium fluoride.

Interaction of nucleotides with Asp351 and the conserved phosphorylation loop of sarcoplasmic reticulum Ca2+-ATPase.

Interaction of tamoxifen with the multidrug resistance p-glycoprotein.

Internal duplication and homology with bacterial transport proteins in the mdr1 (P glycoprotein) gene from multi drug-resistant human cells.

Introduction: Multidrug resistant proteins.

Kinetics of the multidrug transporter (p-glycoprotein) and its reversal. Physiological reviews.

Large scale purification of detergent-soluble p-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A and Walker B mutant proteins.

Localization of the iodomycin binding site in hamster P-glycoprotein.

lsoprenylated f1avonoids - a survey.

Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within, or immediately C-terminal to, transmembrane domains 6 and 12.

Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins.

Membrane topology distinguishes a subfamily of the ATP-binding cassette (ABC) transporters.

Membrane vesicles from multidrug-resistant human cancer cells contain a specific 150 - to 170-kDa protein detected by photoaffinity labeling.

Modulation of adriamycin accumulation and efflux by f1avonoids in HCT-15 colon cells. Activation of p-glycoprotein as a putative mechanism.

Molecular basis of preferential resistance to colchicine in multidrug-resistant human cells conferred by

Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals.

Molecular mechanisms of drug resistance.

Molecular modeling of phenothiazines and related drugs as multidrug resistance modifiers: a comparative molecular field analysiS study.

Multidrug resistance transporter P glycoprotein has distinct but interacting binding sites for cytotoxic drugs and reversing agents.

Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily.

Mutations in either nucleotide binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites.

Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity.

Mutations to amino acids located in predicted transmembrane segment 6 (TM6) modulate the activity and substrate specificity of human P glycoprotein.

New purines and purine analogs as modulators of multidrug resistance.

Non-equivalent cooperation between the two nucleotide-binding folds of P-glycoprotein.

Novel multidrug resistance reversal agents.

Nucleotide occlusion in the human cystic fibrosis transmembrane conductance regulator. Different patterns in the two nucleotide binding domains.

Overcoming of vincristine resistance in P388 leukemia in vivo and in vitro through enhanced cytotoxicity of vincristine and vinblastine by verapamil.

Overexpression of a transporter gene in a multi drug-resistant human lung cancer cell line.

Overexpression of multidrug resistance-associated protein (MRP) increases resistance to natural products drugs.

P-glycoprotein and swelling-activated chloride channels. Methods in Enzymo/ogy.

P-glycoprotein expression as a predictor of the outcome of therapy for Neuroblastoma. The New Eng/and

P-glycoprotein function involves conformational transitions detectable by differential immunoreactivity.

The nucleotide binding domains of multidrug resistance-p-glycoproteins

https://open.uct.ac.za/bitstream/11427/2690/1/thesis_hsf_2001_de_wet_h.pdf

The nucleotide binding domains of multidrug resistance-p-glycoproteins

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