Hillerton, J.E.

Hogeveen, H.

Ikuta, K.

Morita, H.

Shinzawa, H.

Tsenkova, R.

Name not available

Near infrared spectroscopy for cow identification and in-vivo mastitis diagnosis

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Near infrared spectroscopy for cow identification and in-vivo 
mastitis diagnosis 
R. Tsenkova1, H. Monta1, H. Shinzawa1, H. Hogeveen2, J. Eric Hillerton3 and K. Ikuta4 
Graduate Schooi of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe, Japan 
2Utrecht University, The Netherlands 
institute for Animal Health, Compton, Newbury, United Kingdom 
4Hyogo Prefecture Awaji Agricultual Institute, Yougi-naka, Yagi, Mihara, Hyogo 656-0402, 
Japan 
With the new trend of robotic milking, quality control of milk and cow's health has 
become very important issue in the dairy industry. In the near future, a multipurpose 
technology will be required for feed back control in the fully automated milking units. 
In this paper, Near Infrared Spectroscopy (NIRS) and new classification method for 
spectral data analysis have been presented for cow identification and in-vivo mastitis 
diagnosis. NIR spectra of Holstein cow's udders were collected in interactance (reflection 
and diffusion) mode with portable spectrophotometer, Fruit Tester 20 (Fantec Co., Ltd.). 
Three spectra were acquired on each udder quarter, before milking, in the barn, by directly 
fitting the fiber probe at the cow's body. After NIR spectral analysis, quarter foremilk samples 
were collected from the scanned cows and submitted for bacterial analysis to obtain 
reference data. Experiments were held for 8 consecutive months where data was collected 
once a month. Spectra from healthy cows and cows with CNS mastitis were analysed in a 
data set of 258 udder tissue spectra from a total of 23 cows, acquired at different lactation 
stages and seasons. Modified SIMCA classification method was applied for spectral data 
analysis. As a result, sensitivity of 70.4 % and specificity of 67.9 % were achieved. In 
another experiment, udder screening was successfully performed with NIR hand held 
instrument at dairy farms located overseas. The results, also, showed feasibility for cow 
identification based on their tissue spectra. 
Mastitis in dairy production 901 


Near infrared spectroscopy for cow identification and in-vivo mastitis diagnosis

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