Raamsdonk, L.W.D., van

English

Wageningen Yield 

 Projectnr.: 672.235.01 
Detection of animal proteins 
 
Project leader: L.W.D. van Raamsdonk 
 
 
 
 
 
 
Report 2006.007 May 2006 
 
 
 
The Wageningen 2005 meeting of the IAG working group Microscopy 
 
 
 
 
 
 
 
edited by: L.W.D. van Raamsdonk 
 
 
 
 
 
 
 
Business Unit: Unit Safety & Health  
Group: Databases, Risk Assesment & Chain Management 
 
 
 
 
 
 
RIKILT - Institute of Food Safety 
Bornsesteeg 45, 6708 PD Wageningen, the Netherlands 
P.O. Box 230, 6700 AE Wageningen, the Netherlands 
Tel:  +31 (0)317 475422 
Fax: +31 (0)317 417717 
Internet: www.rikilt.wur.nl
 Copyright 2006, RIKILT - Institute of Food Safety.  
 
The client is allowed to publish this report in an integral way and to give this report for perusal to a third party. 
Without approval in writing of RIKILT - Institute of Food Safety it is not allowed: 
• to publish this report partial; 
• to use this report or title of this report for setting up calls, to conduct legal procedures, for advertising or 
non-adverting and for acquisition in general; 
• to use the name of RIKILT - Institute of Food Safety apart from author of this report. 
 
 
 
 
 
 
 
 
 
RIKILT Rapportnummer 2006.007 1
CONTENTS   Page 
 
1 IAG MEETING 14-16 JUNE 2005, WAGENINGEN 1 
2 EVALUATION 1 
 2.1 Opinion on 1 
 2.2 Main conclusions: 3 
 
 
ANNEX 1 Agenda 
ANNEX 2 Lectures 
RIKILT Rapportnummer 2006.007 2 
RIKILT Rapportnummer 2006.007 1
1 IAG MEETING 14-16 JUNE 2005, WAGENINGEN 
The section Microscopy of the IAG, originally a German organisation, has now for years an 
international scope. This working group is meant to be a platform for several diverse topics: the 
organisation of ring trials, for discussion of topics in the field of visible light microscopy, to address 
new development, and to develop new methods. It happens to be that the major subjects arise from the 
field of feed analysis. 
The members of the working group organises at turn the annual meeting, preferably in June. These 
meetings cover two days and a half, with emphasis on the results of the annually organised ring trials, 
current topics and new or improved methods. A second one day meeting is usually organised in October 
and held in Hamburg. The emphasis of this meeting is generally on method development. 
In 2004 the Dutch organisation RIKILT Institute of food safety proposes to organise the annual meeting 
in Wageningen. The working group accepted this proposal. About 38 members of the working group 
participated in this meeting. The agenda with the presented lectures is included in Annex 1. 
In the twelve months before the Wageningen meeting, in Autumn 2004, the problem of bone fragments 
in beet pulp arose. First discovered in Ireland, small quantities of bone fragments were found in beet 
pulp parties in the Netherlands and Germany as well. It was to be expected that a substantial part of the 
lectures addressed this problem. The use of meat and bone meal as fertiliser was recognised as source of 
the bone fragments. This consequence of the legal use of meat and bone meal had political 
repercussions as well: a new Directive published in 2005 shortly after the Wageningen meeting allows a 
higher level than the usually applied null tolerance of meat and bone meal in parties of tuber and root 
crops, provided that the producing member state has a proper risk assessment available, including a 
quantitative indication of the maximum allowed level of content (limit). Besides this issue some other 
subjects have been addressed. 
Most presentations of the control activities and research developments are included in this report. In this 
way a new view on the prohibition of animal by-products and its consequences is documented.  
2 EVALUATION  
At the end of the IAG meeting held in Wageningen 30 evaluation forms have been returned. In most but 
not all cases all questions were answered.  
2.1 Opinion on  
1. The information send prior to the meeting (travel information, programme): 
Excellent 8 
Good 8 ; but too late:  6 
Sufficient 10 
Moderate 3 
Not sufficient  
 
2. The quality of the presentations: 
Excellent 11 
Good 18 
Sufficient 1 
Moderate  
Not sufficient  
RIKILT Rapportnummer 2006.007 2 
3. The presented topics and the structure of the programme: 
Excellent 11 
Good 19 
Sufficient 2 
Moderate  
Not sufficient  
 
4. The possibilities to have discussions during the meeting: 
Excellent 14 
Good 14 
Sufficient 2 
Moderate  
Not sufficient  
 
5. The quality of the lab presentations at RIKILT: 
Excellent 20 
Good 8 
Sufficient 1 
Moderate  
Not sufficient  
 
6. Which topics should be addressed in the future: 
Specific botanical impurities (e.g. Datura, etc.) 2 
Quantification of animal proteins 2 
Tolerances 1 
Open declaration in compound feeding stuffs 1 
A short tour in the hosting city 1 
More time to discuss positive samples from each institute in order to learn from 
each other 1 
 
7. Which topics are discussed too extensive: 
none 
 
8. The quality of the conference halls: 
Excellent 9 
Good 20 
Sufficient  
Moderate  
Not sufficient  
 
9. The quality of the catering (coffee/tea, refreshments, lunch): 
Excellent 9 
Good 17 
Sufficient 2 
Moderate 1 
Not sufficient  
 
 
RIKILT Rapportnummer 2006.007 3
Additional remarks: 
handouts should be available before the meeting 
"the best IAG meeting I have ever had" 
A social "happening" at one of the evenings should have been nice, especially for promoting the 
corporate sense of the group. 
2.2 Main conclusions: 
Only in a few cases the judgments “moderate” or “sufficient” were given. In the view that the majority 
of the answers indicates at least “good” this does not seem to be a major criticism.  
While the first announcements and invitation were send in December 2004, the programme and formal 
invitation were send in April 2005, which is rather late for those who need these documents  for getting 
a management permission to attend the meeting. It was, however, impossible to send the programme 
earlier since the proposals for lecture were not received in earlier. The hosting institute changes every 
year and RIKILT was not aware of the need to send a formal invitation until a request to do so was 
received in April.  
RIKILT experienced the problem that the hosting institute had to figure out the necessary activities and 
circumstances, and to make an appropriate schedule, without any experience for organising former IAG 
Meetings. A lot of experiences are available at institutes hosting the meeting in the recent past, but these 
experiences are not contained in a shared information package. A proposal to have a board member 
appointed to keep information necessary for organising meetings, such as recent address lists, action list, 
schedule (expressed in days/weeks prior to meeting) of necessary activities, evaluation of recent 
meetings, etc. This will greatly optimise the work for organising the meeting and avoids confusion.  
It is recommended to have hand-outs already available during the meeting. That means that all 
presentations should be available at least some days (a week) before the start of the meeting. It also 
helps in preparing the computer and smoothes the sessions. 
 
RIKILT Rapportnummer 2005.007 
ANNEX 1 Agenda 
 
Time / place Subject Name 
Tuesday 14-6   
08:45-09:15 Welcome, distribution of symposium folder desk 
09:15-10:15 Opening, introduction of participants and general IAG meeting 
IAG flyer 
Inge Paradies-Severin 
 
Franz Wernitznig 
10:15-10:45 coffee break  
10:45-12:30 Results of the animal Protein ringtest 2005 
Ring test for open declaration 
Result of the ring test on open declaration 
Ring test for fertilizers 
Jan S Jorgenson 
Inge Sloot 
Genevieve Frick 
Franz Wernitznig 
12:30-13:30 Lunch  
13:30-14:45 Ring test for ergot 
Ring trials: general discussion 
Bernd Goelz 
Inge Paradies-Severin 
14:45-15:15 tea break  
15:15-15:50 
15:50-16:30 
Status of the near-infrared microscopy and imaging methods 
Tracing contamination through the feed chain - a practical 
example from Sweden 
Vincent Baeten 
Erik Nordkvist 
Wednesday 15-6   
09:00-09:20 
 
09:20-09:40 
 
09:40-10:05 
10:05-10:25 
Current situation in Germany considering animal constituents in 
sugar beet pulp 
Potential of the microscopical analysis to determine the origin of 
bone fragments in the production process of sugar beet pulp 
Further investigations in bone material picked out of sugar pulp 
Bone particles in sugar beet pulp including some pictures 
Inge Paradies-Severin 
 
Diana Hormisch 
 
Regina Modi 
Britta Hertel 
10:25-10:55 coffee break  
10:55-11:05 
11:05-11:20 
 
11:20-11:40 
11:40:12:30 
Training courses on detection of animal proteins 
Problems concerning material of animal origin found in feed of a 
farm using animal meal for biogas production  
Quantification of animal constituents in feed stuffs 
Animal proteins: general discussion 
Leo van Raamsdonk 
Regina Modi 
 
Leo van Raamsdonk 
Inge Paradies-Severin 
12:30-13:30 Lunch  
13:30-16:00 Laboratory visit at RIKILT Leo van Raamsdonk 
16:00-17:00 Social  
Thursday 16-6   
09:00-09:35 
09:35-10:15 
Botanical impurities 
Report of the development of IAG methods 
Leo van Raamsdonk  
Marion Dunker and Diana 
Hormisch 
10:15-10:45 coffee break  
10:45-12:00 Reading of methods 
general meeting 
Inge Paradies-Severin 
12:00-13:00 farewell lunch  
RIKILT Rapportnummer 2005.007 
ANNEX 2. Lectures 
 
I. Paradies-Severin: Animal constituents in sugar beet pulp: current situation in Germany.  
 
R. Modi, H. Schenkel, J. Breuer: Animal ingredients in sugar beet pulp, further investigations  in bone 
material picked out of a sample. 
 
D. Hormisch: Potential of classical microscopy to determine the entry of bone fragments in sugar beet 
pulp. 
 
B. Hertel: Bone particles in sugar beet pulp. 
 
L.W.D. van Raamsdonk: Quantification of animal proteins in feed (updated version including 
information from the new Directive 1292/2005/EC). 
 
E. Nordqvist: Tracing animal constituents through the feed chain. 
 
R. Modi: Animal ingredients in feed: further problems concerning bone material. 
 
V. Baeten and P. Dardenne: Infrared microscopic method for detection of animal proteins: actual status. 
 
L.W.D. van Raamsdonk: Training courses on detection of animal proteins. 
 
L.W.D. van Raamsdonk: Botanical impurities, microscopic detection. 
 
M. Dunker and D. Hormisch: Stand der Methodenarbeit IAG Mikroskopie.
RIKILT Rapportnummer 2005.007 
I. Paradies-Severin: Animal constituents in sugar beet pulp: current situation in 
Germany.  
RIKILT Rapportnummer 2005.007 
R. Modi, H. Schenkel, J. Breuer: Animal ingredients in sugar beet pulp, further 
investigations  in bone material picked out of a sample. 
RIKILT Rapportnummer 2005.007 
D. Hormisch: Potential of classical microscopy to determine the entry of bone fragments 
in sugar beet pulp. 
RIKILT Rapportnummer 2005.007 
B. Hertel: Bone particles in sugar beet pulp. 
RIKILT Rapportnummer 2005.007 
L.W.D. van Raamsdonk: Quantification of animal proteins in feed (updated version 
including information from the new Directive 1292/2005/EC). 
RIKILT Rapportnummer 2005.007 
E. Nordqvist: Tracing animal constituents through the feed chain. 
 
RIKILT Rapportnummer 2005.007 
R. Modi: Animal ingredients in feed: further problems concerning bone material. 
RIKILT Rapportnummer 2005.007 
V. Baeten and P. Dardenne: Infrared microscopic method for detection of animal 
proteins: actual status. 
RIKILT Rapportnummer 2005.007 
L.W.D. van Raamsdonk: Training courses on detection of animal proteins. 
RIKILT Rapportnummer 2005.007 
L.W.D. van Raamsdonk: Botanical impurities, microscopic detection. 
RIKILT Rapportnummer 2005.007 
M. Dunker and D. Hormisch: Stand der Methodenarbeit IAG Mikroskopie 
 


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