Adams, Ralf H.

Alitalo, Kari

Andreas, Martin

Andresen, Volker

Haegerling, Rene

Kiefer, Friedemann

Nurmi, Harri

Pollmann, Cathrin

Schmidt, Christian

Schulte-Merker, Stefan

PubMed

René Hägerling

Cathrin Pollmann

Martin Andreas

Christian Schmidt

Harri Nurmi

Ralf H Adams

Kari Alitalo

Volker Andresen

Stefan Schulte-Merker

Friedemann Kiefer

Crossref

The EMBO Journal

A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy

Helsingin yliopiston digitaalinen arkisto

During mammalian development, a subpopulation of endothelial cells in the cardinal vein (CV) expresses lymphatic-specific genes and subsequently develops into the first lymphatic structures, collectively termed as lymph sacs. Budding, sprouting and ballooning of lymphatic endothelial cells (LECs) have been proposed to underlie the emergence of LECs from the CV, but the exact mechanisms of lymph vessel formation remain poorly understood. Applying selective plane illumination-based ultramicroscopy to entire wholemount-immunostained mouse embryos, we visualized the complete developing vascular system with cellular resolution. Here, we report emergence of the earliest detectable LECs as strings of loosely connected cells between the CV and superficial venous plexus. Subsequent aggregation of LECs resulted in formation of two distinct, previously unidentified lymphatic structures, the dorsal peripheral longitudinal lymphatic vessel (PLLV) and the ventral primordial thoracic duct (pTD), which at later stages formed a direct contact with the CV. Providing new insights into their function, we found vascular endothelial growth factor C (VEGF-C) and the matrix component CCBE1 indispensable for LEC budding and migration. Altogether, we present a significantly more detailed view and novel model of early lymphatic development. The EMBO Journal (2013) 32, 629-644. doi:10.1038/emboj.2012.340; Published online 8 January 201

Hagerling, R.

Pollmann, C.

Andreas, M.

Schmidt, C.

Nurmi, H.

Adams, R.H.

Alitalo, K.

Andresen, V.

Schulte-Merker, S.

Kiefer, F.

Name not available

NARCIS 

Wageningen Yield 

During mammalian development, a subpopulation of endothelial cells in the cardinal vein (CV) expresses lymphatic-specific genes and subsequently develops into the first lymphatic structures, collectively termed as lymph sacs. Budding, sprouting and ballooning of lymphatic endothelial cells (LECs) have been proposed to underlie the emergence of LECs from the CV, but the exact mechanisms of lymph vessel formation remain poorly understood. Applying selective plane illumination-based ultramicroscopy to entire wholemount-immunostained mouse embryos, we visualized the complete developing vascular system with cellular resolution. Here, we report emergence of the earliest detectable LECs as strings of loosely connected cells between the CV and superficial venous plexus. Subsequent aggregation of LECs resulted in formation of two distinct, previously unidentified lymphatic structures, the dorsal peripheral longitudinal lymphatic vessel (PLLV) and the ventral primordial thoracic duct (pTD), which at later stages formed a direct contact with the CV. Providing new insights into their function, we found vascular endothelial growth factor C (VEGF-C) and the matrix component CCBE1 indispensable for LEC budding and migration. Altogether, we present a significantly more detailed view and novel model of early lymphatic development

http://emboj.embopress.org/content/embojnl/32/5/629.full.pdf

A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy

Abstract

Similar works

Full text

Available Versions

Crossref

Helsingin yliopiston digitaalinen arkisto

Name not available

NARCIS

Wageningen Yield

NARCIS