Malaria-infection in mice results in a wide perturbation of the host serum proteome involving a range of proteins and functions. Of particular interest is the increased secretion of anti-inflammatory and anti apoptotic proteins

Charles RJC Newton

Evelyn N Gitau

Gilbert O Kokwaro

Stephen A Ward

Malaria Journal

PubMed

Gitau, Evelyn

Kokwaro, Gilbert O

Newton, Charles Rjc

Ward, Stephen

LSTM Online Archive

Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry.

Springer - Publisher Connector

Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry

BACKGROUND: A global proteomic strategy was used to identify proteins, which are differentially expressed in the murine model of severe malaria in the hope of facilitating future development of novel diagnostic, disease monitoring and treatment strategies. METHODS: Mice (4-week-old CD1 male mice) were infected with Plasmodium berghei ANKA strain, and infection allowed to establish until a parasitaemia of 30% was attained. Total plasma and albumin depleted plasma samples from infected and control (non-infected) mice were separated by two-dimensional gel electrophoresis (2-DE). After staining, the gels were imaged and differential protein expression patterns were interrogated using image analysis software. Spots of interest were then digested using trypsin and the proteins identified using matrix-assisted laser desorption and ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and peptide mass fingerprinting software. RESULTS: Master gels of control and infected mice, and the corresponding albumin depleted fractions exhibited distinctly different 2D patterns comparing control and infected plasma, respectively. A wide range of proteins demonstrated altered expression including; acute inflammatory proteins, transporters, binding proteins, protease inhibitors, enzymes, cytokines, hormones, and channel/receptor-derived proteins. CONCLUSIONS: Malaria-infection in mice results in a wide perturbation of the host serum proteome involving a range of proteins and functions. Of particular interest is the increased secretion of anti-inflammatory and anti apoptotic proteins

Gitau, EN

Kokwaro, GO

Newton, CR

Ward, SA

Oxford University Research Archive (ORA)

Crossref

Malaria-infection in mice results in a wide perturbation of the host serum proteome involving a range of proteins and functions. Of particular interest is the increased secretion of anti-inflammatory and anti apoptotic proteins.</p

Gitau, Evelyn N.

Kokwaro, Gilbert O.

Ward, Steve

LSTM Research Portal

English

Newton, Charles

Oxford University Research Archive

AG: Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell Microbiol

Bandyopadhyay U: Apoptosis in liver during malaria: role of oxidative stress and implication of mitochondrial pathway.

Cerebral malaria in inbred mice. I. A new model and its pathology. Trans R Soc Trop Med Hyg

Cerebral malaria: role of microparticles and platelets in alterations of the blood-brain barrier.

Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature

Das DB: Plasma alpha-tocopherol, retinol, and carotenoids in children with falciparum malaria.

de Souza JB: Immunopathology of cerebral malaria: morphological evidence of parasite sequestration in murine brain microvasculature. Infect Immun

Ehrhardt W: Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G250 and R-250. Electrophoresis

Ghassabeh GH: Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation.

GN: Molecular pathogenesis of hepatitis B virus infection: simultaneous detection of viral DNA and antigens in paraffinembedded liver sections. Lancet

Hunt NH: Pathology of fatal and resolving Plasmodium berghei cerebral malaria in mice. Parasitology

Immunization of rats against malaria: a new model.

Improvement and simplification of low-background silver staining of proteins by using sodium dithionite. Electrophoresis

KF: Serum retinol concentrations and Schistosoma mansoni, intestinal helminths, and malarial parasitemia: a cross-sectional study in Kenyan preschool and primary school children.

Korneluk RG: Genomic characterization of the mouse inhibitor of apoptosis protein 1 and 2 genes. Genomics

Lal AA: Rapid onset of malariainduced mortality by immunizations with lipo-peptides: an experimental model to study deleterious immune responses and immunopathology in malaria. Vaccine

Mazier D: The role of interleukin-6 in vitamin A deficiency during Plasmodium falciparum malaria and possible consequences for vitamin A supplementation. Immunology

NH: Comparisons between microvascular changes in cerebral and non-cerebral malaria in mice, using the retinal whole-mount technique. Parasitology

Overexpression, purification and localization of Gitau et al.

Pied S: Self-reactivities to the nonerythroid alpha spectrin correlate with cerebral malaria in Gabonese children. PLoS ONE

Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis

Proteomic profiling from human samples: the body fluid alternative. Toxicol Lett

Raynes JG: Influence of morbidity on serum retinol of children in a community-based study in northern Ghana.

Singkamani R: The acute phase response and vitamin A status in malaria. Trans R Soc Trop Med Hyg

Structure of the chromosomal gene for human serum prealbumin. Gene

The acute phase response in the rodent.

Ultrastructural changes in the blood-brain barrier of mice infected with Plasmodium berghei. Acta

WB: Breakdown of the bloodbrain barrier in murine cerebral malaria. Parasitology

WHO: Integrated management of childhood illness: conclusions. WHO Division of Child Health and Development. Bull World Health Organ

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3199904

Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry

Abstract

Similar works

Full text

Available Versions

LSTM Online Archive

Springer - Publisher Connector

Oxford University Research Archive (ORA)

Crossref

LSTM Research Portal

Oxford University Research Archive

LSTM Research Portal