Bain, Judith M

Di Pietro, Antonio

Erwig, Lars P

Gow, Neil A R

Schafer, Katja

PLoS ONE

English

PubMed

Fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. Here we investigated phagocytosis of F. oxysporum by J774.1 murine cell line macrophages using live cell video microscopy. Macrophages avidly migrated towards F. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. F. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. Macrophage killing depended on the multiplicity of infection. After engulfment, F. oxysporum inhibited macrophages from completing mitosis, resulting in large daughter cells fused together by means of a F. oxysporum hypha. These results shed new light on the initial stages of Fusarium infection and the innate immune response of the mammalian host

Schäfer, Katja

Bain, Judith M.

Gow, Neil A. R.

Erwig, Lars P.

Repositorio Institucional de la Universidad de Córdoba

Hyphal Growth of Phagocytosed Fusarium oxysporum Causes Cell Lysis and Death of Murine Macrophages

<div>Fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. Here we investigated phagocytosis of F. oxysporum by J774.1 murine cell line macrophages using live cell video microscopy. Macrophages avidly migrated towards F. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. F. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. Macrophage killing depended on the multiplicity of infection. After engulfment, F. oxysporum inhibited macrophages from completing mitosis, resulting in large daughter cells fused together by means of a F. oxysporum hypha. These results shed new light on the initial stages of Fusarium infection and the innate immune response of the mammalian host.</div

Katja Schäfer (528487)

Judith M. Bain (178564)

Antonio Di Pietro (528488)

Neil A. R. Gow (43882)

Lars P. Erwig (598213)

The Francis Crick Institute

Hyphal Growth of Phagocytosed Fusarium oxysporum Causes Cell Lysis and Death of Murine Macrophages

Katja Schäfer

Judith M Bain

Antonio Di Pietro

Neil A R Gow

Lars P Erwig

Directory of Open Access Journals

Hyphal growth of phagocytosed Fusarium oxysporum causes cell lysis and death of murine macrophages.

Judith M. Bain

Neil A. R. Gow

Lars P. Erwig

Crossref

The Open University

Hyphal growth of phagocytosed fusarium oxysporum causes cell lysis and death of murine macrophages

https://figshare.com/articles/Hyphal_Growth_of_Phagocytosed_Fusarium_oxysporum_Causes_Cell_Lysis_and_Death_of_Murine_Macrophages/1103533

Hyphal growth of phagocytosed fusarium oxysporum causes cell lysis and death of murine macrophages

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Repositorio Institucional de la Universidad de Córdoba

The Francis Crick Institute

Directory of Open Access Journals

Crossref

The Open University