Isolation and characterization of bacteria from Waste Sugar Mill Arasoe-Kab.Bone As Raw Material Producing Bioplastics Degraded (Poly-??-hydroxybutyrate)

Abstract

Beberapa genera bakteri seperti Alcaligenes, Pseudomonas, Azotobacter, Rhizobium, Azospirilum, dan Micrococcus dari beberapa spesies algae mampu mengakumulasi granula PHB yang diakumulasi oleh sel bakteri yang ditumbuhkan dalam medium yang mengandung konsentrasi karbon tinggi tetapi nitrogen dan fosfatnya terbatas, Salah satu upaya untuk menekan biaya produksi perlu dilakukan penelitian dari sudut pandang mikrobiologi, diantaranya ialah menemukan mikroba unggul baru penghasil PHB atau menemukan substrat yang memiliki nilai ekonomi rendah,\ud misalnya limbah pabrik gula.ABSTRACT\ud Plastic is a material widely used in everyday life but difficult to degrade in the environment that would cause environmental problems. One solution to this problem is to find a plastic material that easily degraded in the environment. Poly-??-hydroxybutyrate (PHB) is a poly-mer which is easily degraded and can be produced by microorganisms especially bacteria under conditions of excess carbon source. This study aims to obtain bacterial isolates from Arasoe sugar factory waste that potentially produce PHB. Isolation of bacteria from sewage plants conducted using SPC method. NA medium with 1% glucose addition were use to grow the isolated bacteria. Qualitative selection methods using reagents Sudan Black were use to selected the isolates. Result showed that PHB-producing bacteria isolated from sugar factory waste Arasoe with SPC method obtained that the highest number of bacterial colonies were found on the ground and the lowest of the dregs. From 43 isolates that had been purified and obtained qualitatively, 11 isolates were able to accumulate PHB