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BIOACTIVITY OF PROTEIN FRACTION IN BROWN ALGAE, Turbinaria decurrens, AS ANTIBACTERIAL AGENT

Abstract

A research on the ability of protein fraction from chocolate algae Turbinaria decurrens in territorial of South Sulawesi to inhibit the growth of Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the whole extract using salting out method with 0-20%, 20-40%, 40-60% and 60-80% ammonium sulphate saturation. Protein was purified by a dialysis method using a selophan membrane. The protein level was determined by a Lowry method, the highest protein concentration, 5.1 mg/mL was found in the 0-20% fraction of the\ud chocolate algae. Antibacterial activity test was performed using a layered jelly diffusion method on the MHA medium. The highest antibacterial activity to Staphylococcus aureus was found in whole extract and the 0-20% fraction with the\ud inhibition diameter of 18.63 mm and 13.30 mm, respectively

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