We thank the Genomic Technologies Facility (GTF) and the Central Imaging Facility (CIF) of the University of Lausanne for expert technical support. We thank Valérie Dénervaud Tendon, Guillaume Germion, Deborah Mühlemann, and Kayo Konishi for technical assistance and John Danku and Véronique Vacchina for ICP-MS analysis. This work was funded by grants from the Swiss National Science Foundation (SNSF), the European Research Council (ERC) to NG and a Human Frontiers Science Program (HFSP) grant to JT and NG. GL and CM were supported by the Agropolis foundation (Rhizopolis) and the Agence Nationale de la Recherche (HydroRoot; ANR-11-BSV6-018). MB was supported by a EMBO long-term postdoctoral fellowship, JEMV by a Marie Curie IEF fellowship and TK by the Japan Society for the Promotion of Sciences (JSPS).Peer reviewedPublisher PD

Alassimone, Julien

Barberon, Marie

Geldner, Niko

Kalmbach, Lothar

Kamiya, Takehiro

Lee, Yuree

Li, Guowei

Maurel, Christophe

Pfister, Alexandre

Roppolo, Daniele

Salt, David E

Takano, Junpei

Vermeer, Joop Em

Yamazaki, Misako

eLife

English

PubMed

International audienceThe endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)--major players of endodermal differentiation--into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane

Vermeer, Joop E M

HAL: Hyper Article en Ligne

A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects.

The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)--major players of endodermal differentiation--into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane

Pfister, A.

Barberon, M.

Alassimone, J.

Kalmbach, L.

Lee, Y.

Vermeer, J.E.

Yamazaki, M.

Li, G.

Maurel, C.

Takano, J.

Kamiya, T.

Salt, D.E.

Roppolo, D.

Geldner, N.

Serveur académique lausannois

Archive Ouverte en Sciences de l'Information et de la Communication

Hal-Diderot

We thank the Genomic Technologies Facility (GTF) and the Central Imaging Facility (CIF) of the University of Lausanne for expert technical support. We thank Valérie Dénervaud Tendon, Guillaume Germion, Deborah Mühlemann, and Kayo Konishi for technical assistance and John Danku and Véronique Vacchina for ICP-MS analysis. This work was funded by grants from the Swiss National Science Foundation (SNSF), the European Research Council (ERC) to NG and a Human Frontiers Science Program (HFSP) grant to JT and NG. GL and CM were supported by the Agropolis foundation (Rhizopolis) and the Agence Nationale de la Recherche (HydroRoot; ANR-11-BSV6-018). MB was supported by a EMBO long-term postdoctoral fellowship, JEMV by a Marie Curie IEF fellowship and TK by the Japan Society for the Promotion of Sciences (JSPS).Peer reviewe

Aberdeen University Research

A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

Alexandre Pfister

Marie Barberon

Julien Alassimone

Lothar Kalmbach

Yuree Lee

Joop EM Vermeer

Misako Yamazaki

Guowei Li

Christophe Maurel

Junpei Takano

Takehiro Kamiya

David E Salt

Daniele Roppolo

Niko Geldner

Crossref

Author response: A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

ProdInra

https://hal.science/hal-01120479v1/document

A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

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