Preclinical Study: Hepatoprotective Effects of Paliasa Capsule on Paracetamol-Induced Liver Damage in Rats

Abstract

Paliasa leaves (Kleinhovia hospita L., Sterculiaceae) use in traditional South Sulawesi medicine for jaundice or hepatitis. Currently, Faculty of Pharmacy has made ??????cooperation UNHAS and TPM for Industrial Scale Production efforts of " ???Paliasa Capsule??? that need preclinical studies. Methods: 60 rats were divided into four groups of 15 animals of each, having free access to food and water ad libitum. Group I (control) was given Na-CMC, Group II were received paracetamol 1 g/kgBB and paliasa capsule 56,6 mg/kgBB one a day, Group III was received paracetamol 1 g/kgBB and paliasa capsule 56,6 mg/kgBB twice a day, and Group IV (negative control) was received paracetamol 1 g/kgBB only. The activities of of aspartate transaminase (AST) and alanine transaminase (ALT) were determined in serum (IFCC method). Superoxide dismutase (SOD), nitric oxide (NO) and glutathione peroxidase (GSH-Px) activity were measured in liver homogenates (ELISA method).Result: ???Capsule paliasa??? as hepatogenerative by decreased levels of AST (36,02%) and ALT (46,11%) and increased levels of GSH-Px (20,44%) and NO (35,59%) compared with negative control on paracetamol-induced hepatotoxicity in rat 1g/kgBW but didn???t been able to increased SOD activity. The effective dose of ???paliasa capsule is one a day for 4 weeks. Conclution: ???Paliasa capsule: suggest as a candidate of hepatogenerative dru