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The use of polyethylen glycol (PEG) as selection agent of callus and plantlet of some sugarcane varieties for drought tolerance

Abstract

An experiment was carried out at Tissue culture laboratory, sugarcane factory in Takalar,\ud aiming to analyze the drought resistant capacity of some sugarcane varieties treated with\ud Polyethylen Glycol (PEG) as selecting agent. The research was arranged according to\ud factorial experiment based on completely randomized design. The first factor of variety\ud consisted by three varieties of R-579, Philipin and Q-81. PEG as the second factor consisted\ud by four concentrations namely 0 g L-1, 10 g L-1, 15 g L-1 dan 20 g L-1.\ud Results of the research indicated that there was a variation of variety parameters in response\ud to PEG at the callus state and their plantlet growth. Variety of R579 performed better callus\ud and plantlet growth in terms of rapid root and shoots formation, on the other hand,\ud Philippine variety has more number of roots and shoots. This phenomenon indicated that\ud variety selection at callus state and plantlet can be performed at the early state, yet\ud evaluation of promising variety need to be continued under acclimatization and field\ud condition

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