We have carried out the largest search for stable particles with fractional electric charge, based on an oil drop method that incorporates a horizontal electric field and upward air flow. No evidence for such particles was found, giving a 95%~C.L. upper limit of 1.15×10−22 particles per nucleon on the abundance of fractional charge particles in silicone oil for 0.18e≤∣Qresidual∣≤0.82e. Since this is the first use of this new method we describe the advantages and limitations of the method